ARANIY FADHILAH, . (2016) DETEKSI INFEKSI ARBOVIRAL PADA SAMPEL AKUT DAN CONVALESENCE RSUDWANGAYA, DENPASAR BALI. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.
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Abstract
Studi ini bertujuan mengidentifikasi introduksi Arbovirus jenis baru yang mungkin menginfeksi sampel terduga infeksi arboviral dari RSUD Wangaya, Denpasar, Bali sebagai usaha membuat data surveillance. Infeksi arbovirus (arthropod-borne virus) akibat DENV, CHIKV, dan JEV, merupakan infeksi viral patogenik dengan gejala klinis serius yang sangat mudah menyebar. Denpasar, Bali adalah tempat yang diestimasi paling heterogen dan menjadi tolak ukur apabila terdapat introduksi viral baru (emerging virus) di Indonesia. Serum darah akut (0-7 hari onset) dan convalescence (>10 hari onset) dikoleksi dan diuji dengan metode RTPCR dan MAC ELISA. Hasil menunjukkan tidak terdapat introduksi arboviral baru pada koleksi sampel. Sebanyak 23.07% kasus terinfeksi oleh Flavivirus endemik Indonesia dan 76.93 % kasus sisanya yang tidak terinfeksi Arbovirus dan merupakan indikasi terdapat infeksi lain selain Arbovirus pada spesimen tersebut. Metode RT-PCR dan MAC ELISA sebagai metode deteksi materi genetik virus dan presensi antibodi spesifik virus dapat digunakan dan berperan besar dalam membantu penegakkan keakuratan diagnosis. Data surveillance penderita infeksi arboviral di Bali secara berkala dibutuhkan sebagai indikator distribusi patogenik Arbovirus ke dalam dan luar Indonesia dan dalam membuat dasar peraturan pemberantasan penyakit. This study aims to identify new types of Arbovirus which might be infected samples of suspect arboviral infections from RSUD Wangaya, Denpasar, Bali and as an effort to make the data surveillance. Arboviral infections (arthropod-borne virus) as a result of DENV, CHIKV, and JEV, are a pathogenic viral infection with serious clinical symptoms and are very easily spread. Denpasar, Bali is the place that estimated as the most heterogeneous and become a benchmark if there is a viral introduction (emerging viruses) in Indonesia. Acute blood sera (0-7 days of onset) and convalescence sera (> 10 days of onset) were collected and tested by the method of RT-PCR and ELISA MAC. The results showed that there was no new arboviral introduction to samples collection. A total 23.07% of cases were infected by Flavivirus endemic to Indonesia and 76.93% of the remaining cases were not infected by Arbovirus with an indication other pathogenic agents present in the specimen. RT-PCR method and MAC ELISA should be highly considered due to their main role in helping accuracy of diagnosis. Data surveillance of arboviral infections in Bali suggested to be made regularly as an indicator of the distribution of pathogenic Arbovirus inside and outside Indonesia and as a references in making disease control regulations.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Additional Information: | 1) Dr. Rini Puspitaningrum, M. Biomed 2) Dr.Ir.I Made Artika, M.App.Sc |
| Subjects: | Sains > Ilmu Bumi > Biologi |
| Divisions: | FMIPA > S1 Biologi |
| Depositing User: | sawung yudo |
| Date Deposited: | 30 Mar 2022 02:35 |
| Last Modified: | 30 Mar 2022 02:35 |
| URI: | http://repository.unj.ac.id/id/eprint/25515 |
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