ANALISIS FREKUENSI MIKRONUKLEUS PADA LIMFOSIT SEL MONONUKLEAT PENDUDUK DESA BOTTENG SEBAGAI DAERAH RADIASI ALAM TINGGI

DIAH AYU LESTARI, . (2023) ANALISIS FREKUENSI MIKRONUKLEUS PADA LIMFOSIT SEL MONONUKLEAT PENDUDUK DESA BOTTENG SEBAGAI DAERAH RADIASI ALAM TINGGI. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

ABSTRAK. Suatu daerah yang memiliki jumlah paparan radiasi alam melebihi ambang batas yang telah ditentukan untuk diterima masyarakat, dikategorikan sebagai daerah radiasi alam tinggi (High Natural Background Radiation – HNBR). Daerah HNBR dapat ditemui di Desa Botteng. Kerusakan DNA akibat radiasi dapat dideteksi dengan mikronukleus yaitu inti kecil di luar nukleus. Penelitian ini bertujuan untuk mengetahui perbedaan frekuensi mikronukleus antara kelompok HNBR Desa Botteng dengan kelompok kontrol, pada sel mononukleat dan sel binukleat serta untuk mengetahui hubungan antara mikronukleus pada sel mononukleat dengan mikronukleus pada sel binukleat. Metode yang digunakan adalah descriptive analytic pendekatan cross sectional. Mikronukleus diperoleh dari kultur sel darah CBMN-cyt kelompok HNBR Desa Botteng dan kontrol. Hasil uji t pada sel mononukleat didapat frekuensi mikronukleus kelompok HNBR sebesar 9.25 ± 6.34 lebih tinggi (p < 0.05) dibandingkan frekuensi mikronukleus kelompok kontrol sebesar 2.44 ± 1.38. Pada sel binukleat, frekuensi mikronukleus kelompok HNBR sebesar 16.41 ± 5.89 lebih tinggi (p < 0.05) dibandingkan frekuensi mikronukleus kelompok kontrol sebesar 12.78 ± 4.40. Hasil uji linear regresi kelompok gabungan menunjukkan bahwa frekuensi mikronukleus pada sel mononukleat berhubungan secara positif (p < 0.05 ; r = 0.574) dengan frekuensi mikronukleus pada sel binukleat. Radiasi alam tinggi menyebabkan perbedaan dan peningkatan mikronukleus HNBR dibandingkan dengan mikronukleus kontrol baik pada sel mononukleat maupun sel binukleat. Kata kunci: radiasi, radon, limfosit, CBMNcyt assay, mikronukleus. ********* ABSTRACT. An area can have the amount of exposure to natural radiation that exceeds the threshold that has been determined to be accepted by inhabitants, which is categorized as High Natural Background Radiation (HNBR) area. The HNBR area can be found in Botteng Village. DNA damage due to radiation can be detected by the micronucleus, a small nucleus outside the nucleus. This study aimed to determine the difference between micronucleus frequencies of HNBR group of Botteng Village and control group, in mononucleated cells and binucleated cells and to determine correlation between micronucleus in mononucleated cells and micronucleus in binucleated cells. The method used is descriptive analytic with cross sectional approach. Micronucleus were obtained from CBMN-cyt blood cell culture of HNBR group of Botteng inhabitants and control group. The results of t-test on mononucleated cells showed that micronucleus frequencies of the HNBR group (9.25 ± 6.34) were higher (p < 0.05) compared to micronucleus frequencies of control group (2.44 ± 1.38). In binucleated cells, micronucleus frequencies of the HNBR group (16.41 ± 5.89) were higher (p < 0.05) compared to micronucleus frequencies of control group (12.78 ± 4.40). The linear regression test result showed that micronucleus frequencies of combined group in mononucleated cells was positively correlated (p < 0.05 ; r = 0.574) with micronucleus frequencies in binucleated cells. High natural radiation caused difference and increase the number of HNBR micronucleus compared to control micronucleus in both mononucleated cells and binucleated cells. Keywords : radiation, radon, lymphocyte, CBMNcyt assay, micronucleus

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Yulia Irnidayanti, M.Si. ; 2). Dwi Ramadhani, M.Si.Med.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 17550 not found.
Date Deposited:	07 Mar 2023 06:53
Last Modified:	07 Mar 2023 06:53
URI:	http://repository.unj.ac.id/id/eprint/38108

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