KAJIAN HISTOPATOLOGIS TESTIS MENCIT PASCA SAPIH YANG MENGALAMI DIABETES DAN DIBERI EKSTRAK DAUN KELOR (MORINGA OLEIFERA L.)

MUHAMMAD INDRA SYAHBANNA BARKAH ILMA, . (2023) KAJIAN HISTOPATOLOGIS TESTIS MENCIT PASCA SAPIH YANG MENGALAMI DIABETES DAN DIBERI EKSTRAK DAUN KELOR (MORINGA OLEIFERA L.). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Penyakit metabolik yang disertai hiperglikemia dan diakibatkan oleh kelainan pada sekresi insulin, kinerja insulin, ataupun keduanya dikenal sebagai diabetes melitus. Kondisi diabetes melitus dapat menyebabkan gangguan pada sistem reproduksi pria. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh dari pemberian ekstrak daun kelor terhadap kadar glukosa darah, bobot badan dan testis, jumlah sel spermatogenik, diameter tubulus seminiferus, dan tebal epitel seminiferus mencit pasca sapih diabetes. Penelitian ini menggunakan 5 kelompok perlakuan yaitu Kontrol Sehat (aquades), Kontrol (-) (mencit diabetes + aquades), Kontrol (+) (mencit diabetes + metformin), Kelompok mencit diabetes yang diberi ekstrak daun kelor dosis 200 mg/kgBB, dan Kelompok mencit diabetes yang diberi ekstrak daun kelor dosis 400 mg/kgBB. Parameter yang diamati berupa kadar glukosa darah, bobot badan dan testis, jumlah sel-sel spermatogenik, diameter tubulus seminiferus, dan tebal epitel seminiferus yang dianalisis secara statistik menggunakan ANOVA satu arah dengan α = 0,05. Data yang menunjukkan adanya beda nyata, dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) pada program Statistical Package for the Social Sciences (SPSS). Hasil penelitian menunjukkan bahwa ekstrak daun kelor mampu menurunkan glukosa darah sampai ke taraf normal dengan kadar 104,6 - 110,2 mg/dl, meningkatkan bobot badan sebesar 1,0 - 1,1 gram dan memiliki bobot testis sebesar 74,4 - 79,6 mg, meningkatkan jumlah sel spermatogenik dengan spermatogonium sebesar 40,18 - 41,12 sel, spermatosit sebesar 61,73 – 63,07 sel, dan spermatid sebesar 82,17 - 82,76 sel, memperbaiki diameter tubulus seminiferus dengan nilai 346,73 - 348,75 μm dan tebal epitel seminiferus sebesar 83,04 - 84,23 μm pada mencit pasca sapih diabetes. Dari setiap kelompok perlakuan, terdapat beda nyata pada glukosa darah, bobot badan dan testis, jumlah sel spermatogenik, diameter tubulus seminiferus, serta tebal epitel seminiferus (Sig<0.05). Pemberian ekstrak daun kelor dosis 200 mg/kgBB dan 400 mg/kgBB mampu menurunkan kadar glukosa darah, meningkatkan bobot badan, bobot testis, jumlah sel-sel spermatogenik, serta memperbaiki struktur tubulus seminiferus pada mencit pasca sapih diabetes. *********** Metabolic disease accompanied by hyperglycemia and caused by abnormalities in insulin secretion, insulin performance, or both is known as diabetes mellitus. The condition of diabetes mellitus can cause disturbances in the male reproductive system. The purpose of this study was to determine the effect of moringa leaf extract administration on blood glucose levels, body weight and testes, the number of spermatogenic cells, the diameter of the seminiferous tubules, and the thickness of the seminiferous epithelium in post-weaning diabetes mice. This study used 5 treatment groups, namely Healthy Control (aquades), Control (-) (diabetic mice + distilled water), Control (+) (diabetic mice + metformin), Diabetic mice group given Moringa leaf extract at a dose of 200 mg/kgBB, and the diabetic mice group were given Moringa leaf extract at a dose of 400 mg/kgBB. Parameters measured were blood glucose levels, body and testis weight, number of spermatogenic cells, diameter of the seminiferous tubules, and thickness of the seminiferous epithelium which were statistically analyzed using one-way ANOVA with α = 0.05. Data showing significant differences were followed by the Duncan Multiple Range Test (DMRT) in the Statistical Package for the Social Sciences (SPSS) program. The results showed that Moringa leaf extract was able to reduce blood glucose to a normal level with levels of 104,6 – 110,2 mg/dl, increase body weight by 1,0 – 1,1 grams and have testicular weights of 74,4 – 79,6 mg, increased the number of spermatogenic cells with spermatogonia by 40,18 – 41,12 cells, spermatocytes by 61,73 – 63,07 cells, and spermatids by 82,17 – 82,76 cells, improved the diameter of the seminiferous tubules with a value of 346,73 – 348,75 μm and seminiferous epithelium thickness of 83,04 – 84,23 μm in post-weaning diabetic mice. From each treatment group, there were significant differences in blood glucose, body weight and testes, the number of spermatogenic cells, the diameter of the seminiferous tubules, and the thickness of the seminiferous epithelium (Sig<0.05). Administration of moringa leaf extract at doses of 200 mg/kgBB and 400 mg/kg was able to reduce blood glucose levels, increase body weight, testicular weight, the number of spermatogenic cells, and improve the structure of the seminiferous tubules in post-diabetic mice.

Item Type:	Thesis (Sarjana)
Additional Information:	1). drh. Atin Supiyani, M.Si. ; 2). Dr. Elsa Lisanti, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Fisiologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19400 not found.
Date Deposited:	05 Sep 2023 02:11
Last Modified:	05 Sep 2023 02:11
URI:	http://repository.unj.ac.id/id/eprint/40529

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