POTENSI RESVERATROL TEMPE SEBAGAI AGEN RADIOPROTEKTOR: KAJIAN STRES OKSIDATIF DAN HISTOLOGIS HATI—GINJAL MENCIT

MA'RIFATU FIRRIZKY, . (2023) POTENSI RESVERATROL TEMPE SEBAGAI AGEN RADIOPROTEKTOR: KAJIAN STRES OKSIDATIF DAN HISTOLOGIS HATI—GINJAL MENCIT. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

	Text COVER.pdf Download (3MB)
	Text BAB 1.pdf Download (559kB)
	Text BAB 2.pdf Restricted to Registered users only Download (1MB) | Request a copy
	Text BAB 3.pdf Restricted to Registered users only Download (1MB) | Request a copy
	Text BAB 4.pdf Restricted to Registered users only Download (2MB) | Request a copy
	Text BAB 5.pdf Restricted to Registered users only Download (352kB) | Request a copy
	Text DAFTAR PUSTAKA.pdf Download (1MB)
	Text LAMPIRAN.pdf Restricted to Registered users only Download (4MB) | Request a copy

Abstract

*ABSTRAK* --------- Perubahan aktivitas enzim akibat paparan radiasi berdampak buruk pada mekanisme pertahanan antioksidan didalam sel. Hal tersebut dapat terjadi karena adanya peningkatan radikal bebas yang melebihi tingkat yang dapat diatasi oleh mekanisme pertahanan antioksidan alami tubuh dan kondisi ini dikenal sebagai stres oksidatif. Pada saat antioksidan intraseluler tidak mampu mengatasi jumlah radikal bebas yang melebihi batas, diperlukan adanya agen radioprotektan yang mampu bekerja sebagai antioksidan. Resveratrol menjadi salah satu agen radioprotektan yang telah banyak diteliti. Resveratrol pada tempe diduga memiliki jumlah lebih besar sebagai hasil dari proses fermentasi. Penelitian dilakukan untuk mengetahui kemampuan resveratrol tempe dalam menurunkan level biomarker kerusakan oksidatif MDA dan 8-OHdG, meningkatkan level antioksidan SOD, GPx, dan GSH, dan perubahan histologis hati dan ginjal mencit iradiasi gamma. Uji ELISA digunakan dalam mengukur level 8-OHdG, SOD, dan GPx. Metode Wills digunakan dalam mengukur MDA, dan metode Ellman digunakan dalam mengukur GSH. Hasil penelitian menunjukkan bahwa pemberian resveratrol tempe meskipun tidak secara signifikan, namun cenderung menurunkan level biomarker kerusakan oksidatif MDA dan 8-OhdG. Pemberian resveratrol tempe juga menunjukkan bahwa meskipun tidak signifikan, namun cenderung meningkatkan level enzim antioksidan SOD, GPx, dan GSH. Pemberian resveratrol tempe menunjukkan adanya perbedaan histologis hati dan ginjal yang lebih baik pada mencit iradiasi gamma 3 Gy. Hal ini menjadikan resveratrol tempe dapat menjadi salah satu kandidat radioprotektor yang baik. *ABSTRACT* ----------- Changes in enzyme activity due to radiation exposure have a negative impact on the antioxidant defense mechanisms in cells. This can occur due to an increase in free radicals that exceed the level that can be overcome by the body's natural antioxidant defense mechanisms and this condition is known as oxidative stress. When intracellular antioxidants are not able to overcome the amount of free radicals that exceed the limit, it is necessary to have radioprotectant agents that are able to work as antioxidants. Resveratrol is one of the radioprotectant agents that has been widely studied. Resveratrol in tempeh is thought to have a larger amount as a result of the fermentation process. This study was conducted to determine the ability of tempe resveratrol to reduce levels of oxidative damage biomarkers MDA and 8-OHdG, increase levels of antioxidants SOD, GPx, and GSH, and histological changes in the liver and kidney of gamma-irradiated mice. ELISA test is used to measure 8-OHdG, SOD, and GPx levels. The Wills method is used to measure MDA, and the Ellman method is used to measure GSH. The results showed that the administration of resveratrol in tempe, although not significantly, tended to decrease the levels of the biomarkers of oxidative damage MDA and 8-OhdG. The administration of resveratrol in tempeh also showed that although it was not significant, it tended to increase the levels of the antioxidant enzymes SOD, GPx, and GSH. The administration of resveratrol in tempeh showed better histological differences in liver and kidney in 3 Gy gamma irradiated mice. This makes tempeh resveratrol a good radioprotector candidate.

Item Type:	Thesis (Sarjana)
Additional Information:	1) Dr. Yulia Irnidayanti, M.Si. 2) Harry Nugroho Eko Surniyantoro, M.Sc.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Zoologi Sains > Fisiologi Ilmu Kedokteran > Patologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19768 not found.
Date Deposited:	06 Sep 2023 06:02
Last Modified:	06 Sep 2023 06:02
URI:	http://repository.unj.ac.id/id/eprint/41012

Actions (login required)

View Item