PENGARUH PAKAN BERSALUT HERBAL SIRIH–KIPAHIT TERHADAP KELULUSHIDUPAN DAN HISTOPATOLOGIS HATI IKAN LELE (Clarias sp.) YANG DIINFEKSI Aeromonas hydrophila

AGUNG MULYA DARMAWAN, . (2023) PENGARUH PAKAN BERSALUT HERBAL SIRIH–KIPAHIT TERHADAP KELULUSHIDUPAN DAN HISTOPATOLOGIS HATI IKAN LELE (Clarias sp.) YANG DIINFEKSI Aeromonas hydrophila. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Budidaya ikan lele (Clarias sp.) memiliki kendala yang selalu dialami oleh peternak lele yaitu serangan penyakit MAS yang disebabkan bakteri A. hydrophila. Bahan alami saat ini sering diperhatikan kegunaan dan manfaatnya sebagai antibakteri dan immunostimulan salah satunya adalah daun sirih dan daun kipahit. Tujuan penelitian ini adalah melihat pengaruh pemberian ekstrak herbal sirih-kipahit pada pakan terhadap, nafsu makan tingkat kelulushidupan dan histopatologis hati ikan lele yang diinfeksi A. hydrophila. Metode penelitian ini adalah eksperimen in vivo yang terdiri atas 5 kelompok percobaan dengan 3 ulangan yaitu K- (tanpa perlakuan herbal dan injeksi A. hydrophila), K+ (tanpa perlakuan herbal dengan injeksi A. hydrophila), P25 (25 mL/kg pakan), P50 (50 mL/kg pakan), dan P100 (100 mL/kg pakan). Pengamatan nafsu makan ikan dilakukan selama 7 hari setelah ikan diinfeksi A. hydrophila. Pengamatan kelulushidupan dilakukan selama masa pemberian pakan 14 hari dan selama masa uji tantang 7 hari. Pengambilan sampel histopatologis hati dilakukan pada 7 hari pasca infeksi. Hasil penelitian menunjukkan bahwa ikan lele yang terinfeksi dan diberi pakan bersalut herbal sirih kipahit memulihkan nafsu makan lebih cepat dibanding kontrol positif. Setelah uji tantang, kelompok P25 memberikan kelulushidupan tertinggi (55,14%) serta RPS tertinggi (37,39%). Hasil pengamatan histopatologis hati yang diberikan herbal sirih-kipahit tetap menunjukkan adanya kerusakan berupa degenerasi hidropis, nekrosis dan respon imun berupa melanomakrofag. Berdasarkan hasil dapat disimpulkan dosis 25 mL/kg pakan merupakan dosis terbaik yang dapat memberi pengaruh terhadap kelulushidupan ikan lele serta pemberian pakan herbal sirih-kipahit dapat memodulasi respon imun pada jaringan hati ikan lele yang terinfeksi bakteri A. hydrophila. Catfish (Clarias sp.) farming has obstacles that are always experienced by catfish farmers, namely MAS disease caused by A. hydrophila. Natural ingredients are currently often considered for their usefulness and benefits as antibacterial and immunostimulants, one of which is betel leaf and mexican sunflower leaf. The purpose of this study was to see the effect of giving betel-tithonia herbal extract in feed on appetite, survival rate and liver histopathology of catfish infected with A. hydrophila. This research method is an in-vivo experiment consisting of 5 experimental groups with 3 replicates, namely K- (without herbal treatment and injection of A. hydrophila), K+ (without herbal treatment with injection of A. hydrophila), P25 (25 mL/kg feed), P50 (50 mL/kg feed), and P100 (100 mL/kg feed). Observation of fish appetite was carried out for 7 days after fish were infected with A. hydrophila. Survival observations were overseen during the 14-day feeding period and during the 7-day challenge test period. Liver histopathology sampling was conducted at 7 days post-infection. The results showed that catfish that were infected and fed a mixture of betel kipahit herbs recovered their appetite faster than the positive control. While after the challenge test, the P25 group gave the highest survival (55.14%) and the highest RPS (37.39%). Histopathological observations of the liver given herbal betel-tithonia showed an immune response in the form of melanomacrophages. Based on the results, it can be concluded that the dose of 25 mL/kg feed is the best dose that can influence the fish survival and feeding of betel-tihonia herbs can modulate the immune response in the liver tissue of catfish infected with A. hydrophila.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Yulia Irnidayanti, M.Si 2). Dr. Nunak Nafiqoh, M.Sc
Subjects:	Sains > Ilmu Bumi > Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19549 not found.
Date Deposited:	07 Sep 2023 01:19
Last Modified:	07 Sep 2023 01:19
URI:	http://repository.unj.ac.id/id/eprint/41176

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