PROFIL HEMATOLOGIS DAN KADAR MALONDIALDEHID (MDA) SERUM TIKUS PUTIH (Rattus norvegicus) YANG DIBERI OLEH KOMBINASI EKSTRAK KUNYIT (Curcuma domestica), JAHE MERAH (Zingiber officinale), DAN TEMULAWAK (Curcuma zanthorrhiza)

ANNISA FITRIANA, . (2023) PROFIL HEMATOLOGIS DAN KADAR MALONDIALDEHID (MDA) SERUM TIKUS PUTIH (Rattus norvegicus) YANG DIBERI OLEH KOMBINASI EKSTRAK KUNYIT (Curcuma domestica), JAHE MERAH (Zingiber officinale), DAN TEMULAWAK (Curcuma zanthorrhiza). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Kunyit (Curcuma domestica), jahe merah (Zingiber officinale), dan temulawak (Curcuma zanthorrhiza) sudah banyak dikombinasikan oleh masyarakat Indonesia menjadi obat tradisional, namun penelitian mengenai efek dari kombinasi ketiga ekstrak masih terbatas. Profil hematologis dan malondialdehid (MDA) dapat dijadikan sebagai marker dari efek bahan alam terhadap kondisi tubuh. Penelitian ini bertujuan mengetahui pengaruh ekstrak kombinasi (kunyit, jahe merah, dan temulawak) terhadap profil hematologis dan kadar MDA serum pada tikus. Metode penelitian ini adalah eksperimental dengan desain Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan 25 sampel darah yang dibagi menjadi 2 kelompok kontrol (kontrol positif dan kontrol negatif) dan 3 kelompok pemberian rute oral parasetamol 162mg/200gBB dengan ekstrak kombinasi (kunyit, jahe merah, dan temulawak) konsentrasi K1=25%, 40%, 35%; K2=20%, 60%, 20%; dan K3=15%, 45%, 40% dosis 500mg/kg BB selama 20 hari. Pengukuran profil hematologis menggunakan hematology analyzer Nihon Kohden MEK 6550, sedangkan kadar MDA menggunakan metode Wills. Hasil dianalisis menggunakan One way ANOVA pada α = 0.05 dan uji lanjut Duncan. Hasil penelitian menunjukkan jumlah eritrosit pada kelompok perlakuan parasetamol dengan kombinasi ekstrak konsentrasi K2 berbeda nyata dengan kontrol negatif (KK2) berupa pemberian parasetamol 162mg/200gBB saja (sig.<0.05). Kadar hemoglobin, persentase hematokrit, jumlah total leukosit, kadar MDA tidak terdapat beda nyata antar kelompok perlakuan (sig.>0.05). Oleh karena itu dapat disimpulkan, ekstrak kombinasi berbagai konsentrasi selama 20 hari pada penelitian ini berpengaruh terhadap jumlah eritrosit; namun ekstrak kombinasi ini tidak berpengaruh terhadap kadar hemoglobin, persentase hematokrit, jumlah total leukosit, dan kadar MDA. ************ Turmeric (Curcuma domestica), red ginger (Zingiber officinale), and temulawak (Curcuma zanthorrhiza) have been combined by many Indonesian people into traditional medicines, but research on the effects of the combination of the three extracts is still limited. Hematological profiles and malondialdehyde (MDA) can be used as markers of the effects of natural ingredients on body condition. This study aims to determine the effect of combined extracts (turmeric, red ginger, and temulawak) on the hematological profile and serum MDA levels in rats. This research method is experimental with RAL (completely randomized design). This study used 25 blood samples which were divided into 2 control groups (positive control and negative control) and 3 groups given the oral route of paracetamol 162 mg/200 gBB with combined extracts (turmeric, red ginger, and temulawak) concentration of K1=25%, 40%, 35%; K2=20%, 60%, 20%; and K3 = 15%, 45%, 40% at a dose of 500 mg/kg BW for 20 days. Hematological profile measurements used the Nihon Kohden MEK 6550 hematology analyzer, while MDA levels used the Wills method. The results were analyzed using One way ANOVA at α = 0.05 and continued with Duncan's test. The results showed that the number of erythrocytes in the paracetamol treatment group with a combination of K2 concentration extract was significantly different from the negative control (KK2) in the form of a group that was only given paracetamol at a dose of 162 mg/200g BW (sig.<0.05). Hemoglobin levels, hematocrit percentage, total leukocyte count, MDA levels were not significantly different between treatment groups (sig.>0.05). Therefore it can be concluded, the combined extract of various concentrations for 20 days in this study had an effect on the number of erythrocytes; however, this combined extract had no effect on hemoglobin levels, hematocrit percentage, total leukocyte count, and MDA levels.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. ; 2). drh. Atin Supiyani, M.Si.
Subjects:	Sains > Ilmu Bumi > Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19564 not found.
Date Deposited:	25 Sep 2023 23:54
Last Modified:	25 Sep 2023 23:54
URI:	http://repository.unj.ac.id/id/eprint/42964

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