VIABILITAS LIMFOSIT DAN AKTIVITAS TROMBOLITIK MENCIT (Mus musculus) YANG DIINDUKSI EKSTRAK DAUN KALIANDRA (Calliandra calothyrsus) DAN MINYAK TRANS

ALFRIDA SYAAMANDA KHAIRUNNISA, . (2023) VIABILITAS LIMFOSIT DAN AKTIVITAS TROMBOLITIK MENCIT (Mus musculus) YANG DIINDUKSI EKSTRAK DAUN KALIANDRA (Calliandra calothyrsus) DAN MINYAK TRANS. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Penyakit kardiovaskular dapat terjadi karena adanya radikal bebas yang dapat menyebabkan stres oksidatif, dimana radikal bebas lebih besar dibandingkan antioksidan. Tanaman herbal yang dimanfaatkan sebagai antioksidan, yaitu daun kaliandra yang memiliki kandungan senyawa aktif berupa flavonoid, tanin, dan saponin. Tujuan dari penelitian ini untuk mengetahui pengaruh dari ekstrak daun kaliandra terhadap viabilitas limfosit, aktivitas trombolitik, dan indeks limpa pada mencit. Mencit sebanyak 25 ekor strain DDY berumur 2 bulan, jantan dibagi menjadi kontrol placebo (aquadest), kontrol negatif (MT 200 mg/kg BB), perlakuan 1 (MT 200 mg/kg BB dan ekstrak daun kaliandra 2 mg/kg BB), perlakuan 2 (MT 200 mg/kg BB dan ekstrak daun kaliandra 4 mg/kg BB), dan pelakuan 3 (MT 200 mg/kg BB dan ekstrak daun kaliandra 6 mg/kg BB). Metode penelitian yaitu eksperimental dengan desain RAK. Viabilitas limfosit diukur dengan menghitung jumlah limfosit hidup dan limfosit mati, aktivitas trombolitik diukur dengan menimbang berat koagulan darah, indeks limpa diukur dengan menimbang bobot limpa. Penelitian ini dianalisis menggunakan Oneway ANOVA dan uji lanjut Duncan. Hasil menunjukkan viabilitas limfosit dari ekstrak daun kaliandra sebesar 66,9-73,7% tinggi secara signifikan dibandingkan dengan kontrol negatif (Sig <0,05). Aktivitas trombolitik dari ekstrak daun kaliandra 21,4-27,6% tinggi secara signifikan dibandingkan dengan kontrol negatif 
(Sig <0,05). Sedangkan persentase indeks limpa memperoleh hasil yang tidak berpengaruh signifikan dibandingkan dengan kontrol negatif (Sig >0,05). ekstrak daun kaliandra terbukti dapat meningkatkan persentase aktivitas trombolitik dan viabilitas sel limfosit, namun tidak berpengaruh terhadap persentase indeks limpa. ***** Cardiovascular disease occurs due to the presence of free radicals that can cause oxidative stress, where free radicals are greater than antioxidants. Herbal plants that are utilized as antioxidants, namely calliandra leaves which contain active compounds in the form of flavonoids, tannins, and saponins. The purpose of this study was to determine the effect of calliandra leaf extract as lymphocyte viability, thrombolytic activity, and spleen index in mice. There were 25 mice of DDY strain aged 2 months, males were divided into placebo control (aquadest), negative control (MT 200 mg/kg BW), treatment 1 (MT 200 mg/kg BW and calliandra leaf extract 2 mg/kg BW), treatment 2 (MT 200 mg/kg BW and calliandra leaf extract 4 mg/kg BW), and treatment 3 (MT 200 mg/kg BW and calliandra leaf extract 6 mg/kg BW). The research method is experimental with RAK design. Lymphocyte viability was measured by counting the number of live and dead lymphocytes, thrombolytic activity was measured by weighing the weight of blood coagulant, spleen index was measured by weighing the spleen weight. This research analyzed using Oneway ANOVA and Duncan's further test. The results showed that lymphocyte viability of calliandra leaf extract was 66.9-73.7% significantly higher than the negative control (Sig <0.05). Thrombolytic activity of calliandra leaf extract 21.4-27.6% was significantly high compared to the negative control (Sig <0.05). While the percentage of spleen index obtained results that had no significant effect compared to the negative control (Sig >0.05). Calliandra leaf extract proven to increase the percentage of thrombolytic activity and lymphocyte cell viability, but has no effect on the percentage of spleen index.

Item Type:	Thesis (Sarjana)
Additional Information:	1). drh. Atin Supiyani, M.Si. ; 2). Drs. Refirman Djamahar, M.Biomed.
Subjects:	Sains > Fisiologi Ilmu Kedokteran > Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 20083 not found.
Date Deposited:	02 Oct 2023 01:24
Last Modified:	02 Oct 2023 01:24
URI:	http://repository.unj.ac.id/id/eprint/43019

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