PENGARUH RESVERATROL TEMPE TERHADAP HISTOLOGIS DAN LEVEL β-AMILOID KORTEKS SEREBRAL MENCIT IRADIASI GAMMA

PUTRI MEGA AULIA, . (2024) PENGARUH RESVERATROL TEMPE TERHADAP HISTOLOGIS DAN LEVEL β-AMILOID KORTEKS SEREBRAL MENCIT IRADIASI GAMMA. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Radiasi gamma dapat mengionisasi komponen sel dan menghasilkan spesies oksigen reaktif (ROS). Akumulasi ROS menyebabkan peningkatan peroksidasi lipid pada membran yang ditandai dengan pembentukan malondialdehid (MDA), mengganggu keseimbangan antioksidan glutation (GSH) di otak, memicu pembentukan peptida β-amiloid (Aβ) dan merubah histologis korteks serebral mencit. Resveratrol (Res) sebagai senyawa antioksidan diketahui mampu mengurangi dampak radiasi gamma terhadap otak. Resveratrol tempe dikenal sebagai antioksidan dan mempunyai kemampuan dalam melindungi neuron. Penelitian dilakukan untuk mengetahui pengaruh pemberian resveratrol tempe dosis 10 mg/kg berat badan terhadap level MDA, GSH dan Aβ, serta histologis korteks serebral mencit yang diiradiasi gamma dengan dosis 5 Gy. Mencit dibagi menjadi 6 kelompok, yaitu kontrol, iradiasi gamma dengan dosis 5 Gy, resveratrol standar dosis 10 mg/kg berat badan, resveratrol tempe dosis 10 mg/kg berat badan, iradiasi gamma 5 Gy+resveratrol standar dosis 10 mg/kg berat badan dan iradiasi gamma 5 Gy+resveratrol tempe dosis 10 mg/kg berat badan. Resveratrol diberikan secara oral selama 14 hari. Level MDA, GSH, Aβ diukur dan dilakukan pengamatan histologis korteks serebral mencit. Hasil penelitian menunjukkan bahwa resveratrol tempe dosis 10 mg/kg berat badan pasca iradiasi gamma 5 Gy tidak dapat menyebabkan perubahan level MDA dan GSH namun meningkatkan level Aβ dan tidak mempengaruhi perubahan histologis pada korteks serebral mencit iradiasi gamma dosis 5 Gy setelah 14 hari. Gamma radiation can ionize cell components and produce reactive oxygen species (ROS). The accumulation of ROS causes an increase of lipid peroxidation in cell membranes, balance disturbance of glutathione (GSH) in the brain, triggers the formation of β-amyloid peptide (Aβ), and changes the histology of mice's cerebral cortex. Resveratrol (Res) as an antioxidant compound, is known to reduce the impact of gamma radiation on the brain. Resveratrol tempeh is known as an antioxidant and can protect neurons. This research was conducted to determine the effect of resveratrol tempeh at a dose of 10 mg/kg body weight against MDA, GSH, Aβ levels and the histological changes of in the cerebral cortex of gamma-irradiated mice at a dose of 5 Gy. Mice were divided into 6 groups: control, gamma-irradiated at a dose of 5 Gy, standard resveratrol with the dose of 10 mg/kg body weight, tempeh resveratrol with the dose of 10 mg/kg body weight, gamma-irradiated 5 Gy+standard resveratrol 10 mg/kg body weight and gamma-irradiated 5Gy+tempeh resveratrol 10 mg/kg body weight. Resveratrol was given orally for 14 days. MDA, GSH, Aβ of levels were measured and histological changes were observed. Results showed that tempeh resveratrol at a dose of 10 mg/kg body weight did not cause changes of MDA and GSH levels, but increased Aβ levels and did not affect the histological changes in the cerebral cortex of 5 Gy gamma-irradiated mice after 14 days.

Item Type:	Thesis (Sarjana)
Uncontrolled Keywords:	1). Dr. Yulia Irnidayanti, M.Si. 2). Devita Tetriana, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam
Divisions:	FMIPA > S1 Biologi
Depositing User:	PUTRI MEGA AULIA .
Date Deposited:	02 Feb 2024 04:18
Last Modified:	02 Feb 2024 04:18
URI:	http://repository.unj.ac.id/id/eprint/44108

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