EFEK APOPTOSIS EKSTRAK DAUN SIMPOR (Dillenia suffruticosa) ASAL BELITUNG TERHADAP SEL KANKER KOLOREKTAL WiDr

NAZLIHATUNNISA, . (2024) EFEK APOPTOSIS EKSTRAK DAUN SIMPOR (Dillenia suffruticosa) ASAL BELITUNG TERHADAP SEL KANKER KOLOREKTAL WiDr. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Kanker kolorektal merupakan kanker mematikan urutan kedua di dunia. Efek samping dan resistensi obat kemoterapi yang ada melatarbelakangi berbagai penelitian untuk mencari ekstrak tanaman yang potensial sebagai agen antikanker baru. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksisitas ekstrak daun simpor asal Belitung dan efeknya terhadap apoptosis sel kanker kolorektal WiDr. Kandungan senyawa pada ekstrak daun simpor dianalisa dengan Gas Chromatography-Mass Spectrometry (GC-MS). Aktivitas sitotoksisitas ekstrak daun simpor pada konsentrasi berbeda (25, 50, dan 100 ppm) ditentukan dengan uji MTT dan induksi apoptosis dengan analisa flow cytometry Annexin V/PI. Berdasarkan hasil kromatogram GC-MS didapatkan 13 senyawa berbeda yang terdeteksi. Analisis hubungan konsentrasi ekstrak terhadap persentase inhibisi sel WiDr menunjukkan bahwa konsentrasi ekstrak 100 ppm merupakan konsentrasi dengan persentase inhibisi tertinggi (20,12% ± 4,99) dibandingkan konsentrasi 50 ppm (13,95% ± 2,32) dan 25 ppm (15,90% ± 1,38) dengan nilai konsentrasi inhibitor (IC50) ekstrak daun simpor sebesar 10,45 ppm. Induksi apoptosis sel WiDr oleh ekstrak dipengaruhi oleh konsentrasi ekstrak daun simpor, dimana terjadi peningkatan persentase sel apoptosis dari 16% pada konsentrasi 25 ppm menjadi 70,5% pada konsentrasi 100 ppm. Hasil penelitian ini menunjukkan bahwa ekstrak daun simpor asal Belitung mampu menginduksi apoptosis pada sel kanker kolorektal WiDr. Colorectal cancer is the second deadliest cancer in the world. The side effects and resistance of existing chemotherapy drugs are the background for various studies looking for potential plant extracts as new anticancer agents. This study aims to determine the cytotoxic activity of simpor leaf extract from Belitung and its effect on apoptosis of WiDr colorectal cancer cells. The compound content in simpor leaf extract was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxic activity of simpor leaf extract at different concentrations (25, 50, and 100 ppm) was determined by the MTT test and apoptosis induction by Annexin V/PI flow cytometry analysis. Based on the GC-MS chromatogram results, 13 different compounds were detected. Analysis of the relationship between extract concentration and the percentage of WiDr cell inhibition showed that an extract concentration of 100 ppm was the concentration with the highest percentage of inhibition (20,12% ± 4,99) compared to concentrations of 50 ppm (13,95% ± 2,32) and 25 ppm (15,90% ± 1,38) with an inhibitory concentration value (IC50) of simpor leaf extract of 10,45 ppm. Induction of WiDr cell apoptosis by the extract was influenced by the concentration of simpor leaf extract, where there was an increase in the percentage of apoptotic cells from 16% at a concentration of 25 ppm to 70,5% at a concentration of 100 ppm. The results of this study show that simpor leaf extract from Belitung is able to induce apoptosis in WiDr colorectal cancer cells.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, S.Kep., M.Biomed. ; 2). Ratih Rinendyaputri, S.K.H., M.Biomed.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi Sains > Mikro Biologi > Biokimia
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 21805 not found.
Date Deposited:	02 Feb 2024 05:09
Last Modified:	02 Feb 2024 05:09
URI:	http://repository.unj.ac.id/id/eprint/44251

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