OPTIMASI PERTUMBUHAN ANGGREK SELOP (PAPHIOPEDILUM GLAUCOPHYLLUM J.J. SM) DENGAN PENAMBAHAN EKSTRAK PISANG AMBON DAN PENGGUNAAN WARNA LAMPU LED SECARA IN VITRO

TATU MUTIARA SYIFA, . (2024) OPTIMASI PERTUMBUHAN ANGGREK SELOP (PAPHIOPEDILUM GLAUCOPHYLLUM J.J. SM) DENGAN PENAMBAHAN EKSTRAK PISANG AMBON DAN PENGGUNAAN WARNA LAMPU LED SECARA IN VITRO. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Paphiopedilum glaucophyllum atau anggrek selop merupakan anggrek yang termasuk dalam kategori endangered species pada IUCN Red List of Threatened Species, dan terdaftar dalam Apendiks CITES sehingga P. glaucophyllum termasuk anggrek yang dilarang di perdagangan secara internasional. Penelitian ini dilakukan bertujuan untuk mengetahui konsentrasi ekstrak pisang ambon dan warna lampu LED yang optimal untuk optimasi pertumbuhan serta kadar klorofil plantlet P. glaucophyllum. Penelitian ini dilakukan dengan Rancangan Acak Kelompok dengan subkultur plantlet P. glaucophyllum dilakukan ke enam jenis media perlakuan; kontrol negatif (MS tanpa penambahan ekstrak pisang), kontrol positif (MS, 1 mg/L IAA dan 5 mg/L Kinetin), MS dengan ekstrak pisang (25; 50; 75; 100 g/L) dikombinasikan dengan empat jenis lampu LED; merah (M), biru (B), merah-biru (MB) dan putih (P). Parameter yang diambil mencakup karakter pertumbuhan tanaman, waktu muncul tunas serta kadar klorofil a dan b. Pengamatan dilakukan selama 12 Minggu Setelah Tanam (MST), dan kadar klorofil diukur pada 12 MST. Analisis data dilakukan dengan Uji T, Two-Way Anova dan uji lanjut DMRT bagi perlakuan dengan hasil berpengaruh nyata (signifikansi < 0,05) dengan SPSS ver. 22. Pertumbuhan tinggi tanaman mencapai hasil optimal pada kombinasi ekstrak pisang sebanyak 100 g/L dengan LED berwarna biru. Peningkatan panjang daun yang paling efektif terlihat pada penggunaan pencahayaan LED berwarna merah. Sementara itu, peningkatan jumlah daun yang optimal terlihat pada perlakuan kombinasi 1 mg/L IAA dan 5 mg/L kinetin dengan penerangan LED berwarna putih. Untuk peningkatan jumlah akar yang optimal, kombinasi 1 mg/L IAA dan 5 mg/L kinetin dengan LED berwarna merah memberikan hasil optimal. Peningkatan jumlah tunas yang paling banyak terjadi pada pencahayaan LED berwarna biru, dengan tunas muncul paling cepat ketika menggunakan ekstrak pisang sebanyak 25 g/L. Kadar klorofil paling tinggi ditemukan pada kombinasi LED warna merah dengan 50 g/L ekstrak pisang. Paphiopedilum glaucophyllum, or slipper orchid, is an orchid that is included in the category of endangered species on the IUCN Red List of Threatened Species and is listed in the CITES Appendix, so P. glaucophyllum is an orchid that is prohibited in international trade. This study was conducted to determine the optimal concentration of ambon banana extract and LED lights for growth optimization and chlorophyll levels of P. glaucophyllum plantlets. This study was conducted by Group Randomized Design with P. glaucophyllum plantlet subcultures carried out to six types of treatment media: negative control (MS without addition of banana extract), positive control (MS, 1 mg/L IAA and 5 mg/L Kinetin), MS with banana extract (25; 50; 75; 100 g/L) combined with four types of LED lights; red (M), blue (B), red-blue (MB) and white (P). The parameters include plant growth characteristics,[TS1] bud emergence time, and chlorophyll a and b content. Observations were made for 12 weeks after planting (WAP), and chlorophyll levels were measured at 12 WAP. Data analysis was conducted with T-Test, Two-Way ANOVA, and DMRT for treatment with natural effect results (significance < 0.05) with SPSS ver. 22. The optimal plant height growth is achieved with a combination of banana extract at a concentration of 100 g/L and blue LED. The most effective increase in leaf length is observed when using a red LED. Meanwhile, the optimal increase in the number of leaves is evident in the treatment combination of 1 mg/L IAA and 5 mg/L kinetin with white LED. Combining 1 mg/L IAA and 5 mg/L kinetin with red LED provides the best results for optimal root growth. The highest increase in the number of shoots occurs with blue LED lighting, and shoots emerge most rapidly when using 25 g/L of banana extract. The highest chlorophyll levels were found in the combination of red LED with 50 g/L of banana extract.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Reni Indrayanti, M.Si. ; 2). Popi Aprilianti, M.Si.
Subjects:	Sains > Botani
Divisions:	FMIPA > S1 Biologi
Depositing User:	Tatu Mutiara Syifa .
Date Deposited:	02 Feb 2024 07:04
Last Modified:	02 Feb 2024 07:04
URI:	http://repository.unj.ac.id/id/eprint/44284

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