ISOLASI METABOLIT SEKUNDER EKSTRAK ETIL ASETAT DAUN MANGGIS (Garcinia mangostana) SERTA UJI BIOAKTIVITAS SEBAGAI INHIBITOR TIROSIN KINASE DAN ANTIOKSIDAN

BINA PERMANA, . (2024) ISOLASI METABOLIT SEKUNDER EKSTRAK ETIL ASETAT DAUN MANGGIS (Garcinia mangostana) SERTA UJI BIOAKTIVITAS SEBAGAI INHIBITOR TIROSIN KINASE DAN ANTIOKSIDAN. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Penelitian ini dilakukan dengan tujuan untuk mengisolasi dan menentukan struktur senyawa metabolit sekunder dari ekstrak etil asetat daun manggis (Garcinia mangostana) serta uji bioaktivitasnya sebagai inhibitor tirosin kinase dan antioksidan. Berbagai metode kromatografi digunakan untuk melakukan pemisahan dan pemurnian senyawa. Senyawa 8-deoksigartanin, isolat 2, dan 7β-hidroksi-betulin berhasil diisolasi dari daun manggis dan di karakterisasi menggunakan data spektroskopi UV-Vis, spektrofotometer massa, FT-IR dan NMR (1D dan 2D). Hasil pengujian aktivitas antioksidan menunjukkan bahwa senyawa 8-deoksigartanin dan isolat 2 merupakan antioksidan yang sangat kuat dengan nilai IC50 sebesar 20,04 dan 32,63 μg/mL, sedangkan senyawa 7β-hidroksibetulin tidak aktif sebagai antioksidan karena memiliki IC50 sebesar 1578,1 μg/mL. Senyawa 8-deoksigartanin memiliki potensi sebagai inhibitor tirosin kinase dengan persen penghambatan yang sedang terhadap reseptor EGFR dan KDR masing-masing sebesar 51% dan 78%. Namun tergolong lemah terhadap reseptor HER2 dengan penghambatan sebesar 8%, HER4 sebesar 15%, IGF1R sebesar 13%, InsR sebesar 34%, PDGFRα sebesar 5%, dan PDGFRβ sebesar 14%. Kesimpulan dari penelitian ini adalah dua senyawa golongan santon yaitu 8-deoksigartanin dan isolat 2 serta satu senyawa golongan triterpen yaitu 7β-hidroksibetulin berhasil diisolasi dari ekstrak etil asetat daun manggis, dimana 8-deoksigartanin menunjukkan aktivitas yang sangat kuat sebagai antioksidan dan persen penghambatan yang sedang sebagai inhibitor tirosin kinase terhadap reseptor EGFR dan KDR. Kata Kunci : Antioksidan, Garcinia mangostana, tirosin kinase ****** This study was conducted with the aim of isolating and determining the structure of secondary metabolite compounds from ethyl acetate extract of mangosteen leaves (Garcinia mangostana) and testing their bioactivity as tyrosine kinase inhibitors and antioxidants. Various chromatography methods were used to separate and purify the compounds. Compounds 8-deoxygartanin, isolate 2, and 7β-hydroxy-betulin were successfully isolated from mangosteen leaves and characterized using UV-Vis spectroscopy, mass spectrophotometer, FT-IR and NMR (1D and 2D) data. The results of antioxidant activity testing showed that compounds 8-deoxygartanin and isolate 2 were very strong antioxidants with IC50 values of 20.04 and 32.63 μg/mL, while compound 7β-hydroxy-betulin was not active as an antioxidant because it had an IC50 of 1578.1 μg/mL. The compound 8-deoxygartanin has the potential as a tyrosine kinase inhibitor with moderate inhibition percentages against the EGFR and KDR receptors of 51% and 78%, respectively. However, it is relatively weak against HER2 receptors with inhibition of 8%, HER4 of 15%, IGF1R of 13%, InsR of 34%, PDGFRα of 5%, and PDGFRβ of 14%. The conclusion of this study is that two compounds of the xanthone group, namely 8-deoxygartanin and isolate 2 and one compound of the triterpene group, namely 7β-hydroxy-betulin, were successfully isolated from the ethyl acetate extract of mangosteen leaves, where 8-deoxygartanin showed very strong activity as an antioxidant and a moderate percentage of inhibition as a tyrosine kinase inhibitor against the EGFR and KDR receptors. Keywords : Antioxidant, Garcinia mangostana, tyrosine kinase

Item Type:	Thesis (Sarjana)
Additional Information:	1) Dr. Fera Kurniadewi, M. Si. 2) Dr. Hanhan Dianhar, M. Si.
Subjects:	Sains > Kimia
Divisions:	FMIPA > S1 Kimia
Depositing User:	Bina Permana .
Date Deposited:	02 Aug 2024 04:39
Last Modified:	02 Aug 2024 04:39
URI:	http://repository.unj.ac.id/id/eprint/48023

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