ADELIA NISRINA KHANZA, . (2022) VALIDASI METODE ANALISIS FOSFOR PADA DAGING DENGAN PEREDUKSI HYDRAZINE SULFATE MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.
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Abstract
Fosfor terkandung dalam pakan hewan yang biasa dikonsumsi untuk pemenuhan nutrisi hewan ternak. Di dalam pakan ternak terdapat fosfor yang akan menyebabkan akumulasi fosfor pada daging. Analisis fosfor diukur dengan menggunakan spektrofotometri UV-Vis dengan menentukan kondisi optimum analisis fosfor kemudian dilanjutkan validasi metode analisis yang terdiri dari parameter linieritias, sensitivitas, batas deteksi dan batas kuantisasi, akurasi dan presisi. Reaksi molibdenum biru menghasilkan senyawa kompleks fosfomolibdat yang memiliki panjang gelombang 689 nm. Hasil validasi metode analisis fosfat yang direduksi hidrazin sulfat memiliki linieritas dengan R2=0,9984 pada rentang 0-0,05 mg L-1. Absorptivitas molar sebesar 7,9259 x 104 L mol-1 cm-1. Serta batas deteksi sebesar 5,26 x 10-4 mg L-1 dan batas kuantisasi sebesar 1,59 x 10-3 mg L-1. Nilai presisi dinyatakan sebagai persen standar deviasi relative %RSD pada bebek, ayam dan sapi masing – masing sebesar 1.,3%, 1,34% dan 1,08%. Perolehan kembali didapatkan masing – masing sampel daging berkisar 90%-117%. Fosfat yang terkandung dalam daging bebek, ayam dan sapi tidak melebihi baku mutu menurut Persatuan Ahli Gizi Indonesia (PERSAGI). ******************************************** Animal feed which is usually consumed to maintain the nutrition of livestock contains phosphor. The phosphor in animal feed may cause an accumulated phosphor in meat. Therefore, a regular phosphor analysis is needed to make sure the food is safe. Phosphor analysis is measured by using spectrophotometry UV-Vis method by determining the optimum conditions for phosphorus analysis and then continued analysis validation which consists of linearity parameter, sensitivity, limit of detection and quantitation, accuracy, and precision. The reaction of blue molybdenum results in complex phosphomolybdate compound with a wavelength of 689 nm. The method validation of analysis of phosphate reduced hydrazine sulfate have a linearity of the method with R2=0.9984 in the range of 0 - 0.05 mg L-1. The molar absorptivity of 7.9259 x 104 L mol-1 cm-1. The value of limit of detection (LOD) of 5.26 x 10-4 mg L-1 and quantitation limit (LOQ) value of 1.59 x 10-3 mg L-1. The precision values were expressed as percent of the relative standard deviations of %RSD in duck, chicken and cow of 1.33%, 1.34% and 1.08%. The percent recovery is obtained for each sample of meat ranged from 90% - 117%. Phosphate contained in duck, chicken and cow did not exceed the quality standard according to the Indonesian Nutritionist Association (PERSAGI).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Additional Information: | 1). Dr. Zulhipri, M.Si. ; 2). Yussi Pratiwi, M.Sc |
| Subjects: | Sains > Kimia |
| Divisions: | FMIPA > S1 Kimia |
| Depositing User: | sawung yudo |
| Date Deposited: | 21 Oct 2024 07:16 |
| Last Modified: | 21 Oct 2024 07:16 |
| URI: | http://repository.unj.ac.id/id/eprint/51737 |
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