OPTIMASI METODE STERILISASI EKSPLAN DAN PENGARUH BAP (Benzyl Amino Purine) TERHADAP PERTUMBUHAN Hoya diversifolia Blume DAN Hoya phyllura O.Schwartz SECARA IN VITRO

YAYU, . (2025) OPTIMASI METODE STERILISASI EKSPLAN DAN PENGARUH BAP (Benzyl Amino Purine) TERHADAP PERTUMBUHAN Hoya diversifolia Blume DAN Hoya phyllura O.Schwartz SECARA IN VITRO. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Hoya adalah jenis tumbuhan epifit merambat dan termasuk tumbuhan dikotil asal Asia Tenggara. Hoya memiliki berbagai potensi yaitu sebagai tanaman hias, penyerap polutan, dan obat yang perlu dikembangkan dan dimanfaatkan lebih luas. Tujuan dari penelitian ini untuk mengetahui teknik sterilisasi yang optimal, pengaruh BAP (Benzyl Amino Purine) terhadap pertumbuhan hoya, perbedaan hasil dari perlakuan sterilisasi dan BAP yang diberikan antara Hoya diversifolia dan Hoya phyllura secara in vitro. Penelitian ini dilakukan dengan desain Rancangan Acak Lengkap (RAL). Eksplan yang digunakan berupa eksplan batang bernodus yang diberi perlakuan sterilisasi yaitu sterilisasi X (Clorox secara berkala 50% ke 20%) dan Y (Clorox secara berkala 30% ke 20%). Eksplan ditanam dalam media ½ MS yang mengandung BAP (0, 1, 2, 3, 4, 5, dan 6) mg/L. Parameter yang diamati di antaranya persentase hidup, persentase kontaminasi, persentase jenis kontaminan, waktu muncul kontaminasi, jumlah akar, panjang akar, dan waktu muncul kalus. Jenis kontaminan juga diamati. Seluruh data diolah dengan menggunakan SPSS 25. Berdasarkan hasil penelitian, teknik sterilisasi optimal H. phyllura terdapat pada perlakuan X (Clorox secara berkala 50% ke 20%), sedangkan H. diversifolia terdapat pada perlakuan X (Clorox secara berkala 50% ke 20%) dan Y (Clorox secara berkala 30% ke 20%) karena keduanya tidak memberikan hasil yang berbeda signifikan. Perlakuan BAP yang ditambahkan tidak berpengaruh terhadap pertumbuhan akar, daun, dan tunas tetapi dapat menumbuhkan kalus. Secara keseluruhan, tidak terdapat perbedaan dari hasil perlakuan pemberian BAP terhadap pertumbuhan antara Hoya diversifolia dan Hoya phyllura.*****Hoya is a type of climbing epiphytic plant and a dicotyledonous plant native to Southeast Asia. Hoya has various potentials, such as being an ornamental plant, a pollutant absorber, and a medicinal plant that needs to be further developed and utilized. This study aimed to determine the optimal sterilization technique, the effect of BAP (Benzyl Amino Purine) on the growth of hoya, and the differences in the results of sterilization and BAP treatments applied between Hoya diversifolia and Hoya phyllura in vitro. The research was conducted using a Completely Randomized Design (CRD). The explants used were nodal stem explants treated with sterilization techniques, namely sterilization X (Clorox gradually reduced from 50% to 20%) and Y (Clorox gradually reduced from 30% to 20%). The explants were cultured on ½ MS medium containing BAP at concentrations of (0, 1, 2, 3, 4, 5, and 6) mg/L. The observed parameters included survival percentage, contamination percentage, types of contaminants, time to contamination appearance, number of roots, root length, and time to callus formation. Contaminant types were also observed. All data were processed using SPSS 25. Based on the results, the optimal sterilization technique for H. phyllura was treatment X (Clorox gradually reduced from 50% to 20%), while for H. diversifolia, both treatments X (Clorox gradually reduced from 50% to 20%) and Y (Clorox gradually reduced from 30% to 20%) were equally effective, as there was no significant difference between them. The BAP treatment applied did not affect root, leaf, and shoot growth but promoted callus formation. Overall, there was no difference in the results of BAP treatments on the growth of H. diversifolia and H. phyllura.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Rizal Koen Asharo, S.Si., M.Si. 2). Prof. Dr. Ir. Sri Rahayu, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Botani
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 26582 not found.
Date Deposited:	24 Feb 2025 07:21
Last Modified:	24 Feb 2025 07:21
URI:	http://repository.unj.ac.id/id/eprint/53369

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