AKTIVITAS ANTIOKSIDAN PADA KOMBINASI KUNYIT (Curcuma domestica Val.), LENGKUAS (Alpinia galanga L.), DAN TEMU KUNCI (Boesenbergia pandurata)

SYIFA PERMATASARI, . (2025) AKTIVITAS ANTIOKSIDAN PADA KOMBINASI KUNYIT (Curcuma domestica Val.), LENGKUAS (Alpinia galanga L.), DAN TEMU KUNCI (Boesenbergia pandurata). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Rimpang Kunyit (Curcuma domestica Val.), lengkuas (Alpinia galanga L.), dan temu kunci (Boesenbergia pandurata) merupakan tanaman obat yang banyak digunakan. Penelitian ini bertujuan untuk menguji aktivitas antioksidan pada kombinasi kunyit, lengkuas, dan temu kunci dengan menggunakan metode DPPH, ABTS, dan FRAP. Metode deskriptif digunakan untuk penapisan fitokimia dan mengidentifikasi jenis senyawa fitokimia berupa flavonoid dan fenol. Metode eksperimental digunakan untuk menganalisis kadar total fenol dan total flavonoid serta aktivitas antioksidan berdasarkan metode DPPH, ABTS, dan FRAP. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktorial. Faktor pertama adalah variasi kombinasi ekstrak yang terdiri dari 3 taraf perlakuan (1:1:1, 1:2:1, 2:1:2). Faktor kedua adalah konsentrasi ekstrak yang terdiri dari 7 taraf perlakuan (10 ppm, 50 ppm, 100 ppm, 150 ppm, 200 ppm, kontrol positif, dan kontrol negatif). Hasil penelitian menunjukkan bahwa kombinasi ekstrak kunyit, lengkuas, dan temu kunci positif mengandung senyawa fenol dan flavonoid. Hasil analisis kuantitatif kadar total fenol dan flavonoid tertinggi pada konsentrasi 100-200 ppm. Kombinasi tersebut mampu meredam radikal DPPH, ABTS, dan FRAP. Hasil optimal metode DPPH pada sampel B dan C (200 ppm) sebesar 71,63% dan 72,90%. Pada metode ABTS, hasil optimal terdapat pada sampel A dan C (200 ppm) dengan peredaman 94,71% dan 95,57%. Metode FRAP, pada sampel B dan C (200 ppm) dengan peredaman 49,74% dan 50,56%. Nilai IC50 terbaik diperoleh pada metode DPPH (sampel B, 61,81 ppm), ABTS (sampel B, 11,38 ppm), dan FRAP (sampel A, 237,50 ppm). ***** Turmeric rhizome (Curcuma domestica Val.), galangal (Alpinia galanga L.), and fingerroot (Boesenbergia pandurata) are widely used medicinal plants. This study aims to test the antioxidant activity of a combination of turmeric, galangal, and fingerroot using the DPPH, ABTS, and FRAP methods. The descriptive method was used for phytochemical screening and to identify the types of phytochemical compounds in the form of flavonoids and phenols. The experimental method is used to analyze the total phenol and total flavonoid content as well as antioxidant activity based on the DPPH, ABTS, and FRAP methods. This study uses a Completely Randomized Design (CRD) with two factors. The first factor is the variation in extract combinations, which consists of 3 treatment levels (1:1:1, 1:2:1, 2:1:2). The second factor is the concentration of the extract, which consists of 7 treatment levels (10 ppm, 50 ppm, 100 ppm, 150 ppm, 200 ppm, positive control, and negative control). The research results show that the combination of turmeric, galangal, and fingerroot extracts positively contains phenolic and flavonoid compounds. The results of the quantitative analysis showed that the highest total phenol and flavonoid content was at a concentration of 100-200 ppm. That combination is capable of neutralizing DPPH, ABTS, and FRAP radicals. The optimal results of the DPPH method for samples B and C (200 ppm) were 71.63% and 72.90%, respectively. In the ABTS method, the optimal results were found in samples A and C (200 ppm) with inhibition rates of 94.71% and 95.57%. FRAP method, in samples B and C (200 ppm) with inhibition of 49.74% and 50.56%. The best IC50 values were obtained using the DPPH method (sample B, 61.81 ppm), ABTS (sample B, 11.38 ppm), and FRAP (sample A, 237.50 ppm).

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. 2). Rizal Koen Asharo, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 27066 not found.
Date Deposited:	28 Feb 2025 03:22
Last Modified:	28 Feb 2025 03:22
URI:	http://repository.unj.ac.id/id/eprint/53864

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