ANALISIS RESPONS GENOTOKSISITAS HEMOSIT KERANG HIJAU (Perna viridis LINNAEUS, 1758) DI SEPANJANG TELUK JAKARTA MENGGUNAKAN UJI MIKRONUKLEUS

RIVALDY ZEIDANE KRISTIANDO, . (2025) ANALISIS RESPONS GENOTOKSISITAS HEMOSIT KERANG HIJAU (Perna viridis LINNAEUS, 1758) DI SEPANJANG TELUK JAKARTA MENGGUNAKAN UJI MIKRONUKLEUS. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Teluk Jakarta mengalami pencemaran logam berat dan mikroplastik akibat aktivitas antropogenik yang tinggi. Kerang hijau (Perna viridis Linnaeus, 1758) sebagai filter feeder berpotensi mengakumulasi polutan dan digunakan sebagai bioindikator dalam studi genotoksisitas. Penelitian ini bertujuan untuk mendeskripsikan morfologi hemosit, menganalisis perbedaan frekuensi mikronukleus dan abnormalitas inti hemosit lainnya antar lokasi, serta hubungan antara parameter morfometrik dengan frekuensi mikronukleus. Sampel diambil dari empat lokasi di Teluk Jakarta, yaitu Muara Kamal, Muara Tahang, Pulau Onrust, serta Pulau Reklamasi, dan dianalisis menggunakan uji mikronukleus. Hasil menunjukkan morfologi hemosit dengan pseudopodia dan abnormalitas inti, meliputi mikronukleus, nuclear buds (NBs), binucleated cells (BNCs), dan nuclear abnormalities (NA) lainnya. Hasil uji Kruskal-Wallis menunjukkan frekuensi mikronukleus, NBs, dan BNCs berbeda signifikan antar lokasi (p <0,05), sedangkan NA lainnya tidak berbeda signifikan (p = 0,140). Frekuensi mikronukleus relatif tinggi tercatat di Muara Kamal (26,16 ± 12,59‰) dan relatif rendah di Pulau Onrust (14,60 ± 5,94‰). Frekuensi NBs relatif tinggi di Pulau Reklamasi (2,43 ± 1,27‰) dan relatif rendah di Pulau Onrust (1,35 ± 0,91‰), sementara frekuensi BNCs relatif tinggi di Muara Kamal (1,87 ± 1,28‰) dan relatif rendah di Pulau Onrust (1,07 ± 0,64‰). Frekuensi NA lainnya relatif tinggi di Pulau Reklamasi (2,75 ± 1,39‰) dan relatif rendah di Muara Tahang (2,12 ± 0,95‰). Terdapat hubungan positif antara panjang, lebar, dan berat tubuh kerang dengan frekuensi mikronukleus (r >0,75; p <0,05). Hasil penelitian ini memperlihatkan respons genotoksisitas hemosit terhadap paparan polutan di Teluk Jakarta. Kata kunci: Genotoksisitas, hemosit, kerang hijau, Teluk Jakarta, uji mikronukleus. ***** Jakarta Bay is contaminated with heavy metals and microplastics due to intense anthropogenic activities. The green mussel (Perna viridis Linnaeus, 1758), as a filter feeder, has the potential to accumulate pollutants and is used as a bioindicator in genotoxicity studies. This study aimed to describe hemocyte morphology, analyze differences in the frequency of micronuclei and other nuclear abnormalities among locations, and assess the relationship between morphometric parameters and micronucleus frequency. Samples were collected from four locations in Jakarta Bay: Muara Kamal, Muara Tahang, Onrust Island, and the Reclamation Island; and analyzed using the micronucleus assay. The results revealed hemocytes exhibiting pseudopodia and nuclear abnormalities, including micronuclei, nuclear buds (NBs), binucleated cells (BNCs), and other nuclear abnormalities (NAs). Kruskal-Wallis test results showed significant differences in the frequency of micronuclei, NBs, and BNCs among locations (p <0.05), while other NAs did not differ significantly (p = 0.140). Relatively high micronucleus frequencies were recorded in Muara Kamal (26.16 ± 12.59‰) and relatively low in Onrust Island (14.60 ± 5.94‰). NBs were relatively higher in Reclamation Island (2.43 ± 1.27‰) and lower in Onrust Island (1.35 ± 0.91‰), whereas BNCs were higher in Muara Kamal (1.87 ± 1.28‰) and lower in Onrust Island (1.07 ± 0.64‰). Other NAs were relatively higher in Reclamation Island (2.75 ± 1.39‰) and lower in Muara Tahang (2.12 ± 0.95‰). A positive correlation was found between shell length, width, and weight and the frequency of micronuclei (r >0.75; p <0.05). These results demonstrated that the genotoxic response of hemocytes to pollutant exposure in Jakarta Bay. Keywords: Genotoxicity, green mussel, hemocyte, Jakarta Bay, micronucleus assay.

Item Type:	Thesis (Sarjana)
Additional Information:	1) Prof. Dr. Yulia Irnidayanti, M.Si. 2) Vina Rizkawati, S.Si., M.Sc.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi Sains > Ilmu Bumi > Genetika Sains > Zoologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 31187 not found.
Date Deposited:	19 Aug 2025 06:30
Last Modified:	19 Aug 2025 06:30
URI:	http://repository.unj.ac.id/id/eprint/61625

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