UJI SITOTOKSISITAS EKSTRAK DAUN SIMPOR (Dillenia suffruticosa Martelli) ASAL BELITUNG TERHADAP CELL LINE MCF-7 DAN HepG2

ARIEF PRASETIYO, . (2021) UJI SITOTOKSISITAS EKSTRAK DAUN SIMPOR (Dillenia suffruticosa Martelli) ASAL BELITUNG TERHADAP CELL LINE MCF-7 DAN HepG2. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Kanker adalah penyakit yang disebabkan oleh sel-sel yang tumbuh secara abnormal. Ekstrak daun simpor memiliki kandungan fitokimia seperti saponin, triterpenoid, sterol, dan polifenol yang bersifat sitotoksik terhadap cell line. Tujuan dari penelitian ini ialah mengetahui kemampuan sitotoksisitas ekstrak daun Dillenia suffruticosa terhadap cell line MCF-7 dan HepG2. Metode yang digunakan adalah eksperimen dengan desain penelitian Rancangan Acak Lengkap (RAL). Kelompok perlakuan terdiri dari ekstrak daun simpor 500, 1.000, 2.000, 5.000 dan 9.000 ppm untuk uji BSLT, ekstrak daun simpor 10, 15, 20, 25 dan 50 ppm untuk uji MTT dan kontrol (tanpa pemberian ekstrak). Data pada uji BSLT ditentukan dengan melihat nilai LC50 yang diuji menggunakan analisis probit, sedangkan uji MTT dianalisis secara statistik menggunakan Anova. Pada uji BSLT hasil yang diperoleh menunjukkan bahwa ekstrak daun simpor tidak bersifat toksik. Namun ketika ekstrak daun simpor diujikan kepada cell line MCF7 dan HepG2 memiliki kemampuan sitotoksisitas. Nilai LC50 sebesar 5,221 ppm. Pemberian ekstrak daun simpor dengan konsentrasi 50 ppm menunjukkan hasil tertinggi terhadap cell line MCF-7 dan konsentrasi 25 ppm menunjukkan hasil tertinggi terhadap cell line HepG2 ***** Cancer is a disease caused by cells that grow abnormally. Simpor leaf extract contains phytochemicals such as saponins, triterpenoids, sterols, and polyphenols which are cytotoxic to cell lines. The purpose of this study was to determine the cytotoxicity of Dillenia suffruticosa leaf extract against cell lines MCF-7 and HepG2. The method used is an experimental research design with Completely Randomized Design (CRD). The treatment group consisted of simpor leaf extract 500, 1.000, 2.000, 5.000 and 9.000 ppm for the BSLT test, simpor leaf extract 10, 15, 20, 25 and 50 ppm for the MTT test and control (without extract). The data on the BSLT test was determined by looking at the LC50 value which was tested using probit analysis, while the MTT test was determined by looking at the IC50 and analyzed statistically using Anova. In the BSLT test the results obtained showed that the simpor leaf extract was not toxic. However, when simpor leaf extract was tested on the cell line MCF-7 and HepG2, it had cytotoxicity. The LC50 value is 5.221 ppm. The administration of simpor leaf extract with a concentration of 50 ppm showed the highest yield to the MCF-7 cell line and a concentration of 25 ppm showed the highest yield to the HepG2 cell line

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns.Sri Rahayu, M. Biomed ; 2). Drh. Atin Supiyani, M. Si.
Subjects:	Sains > Mikro Biologi
Divisions:	FMIPA > S1 Pendidikan Biologi
Depositing User:	sawung yudo
Date Deposited:	08 Jun 2022 06:45
Last Modified:	23 Jan 2024 04:46
URI:	http://repository.unj.ac.id/id/eprint/29882

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