PENAPISAN DAN OPTIMASI PRODUKSI LIPID KHAMIR OLEAGINOUS ASAL FERMENTASI BIJI KAKAO DENGAN VARIASI SUMBER KARBON

ALMIRA MARVELLA PRISKANINGRUM, . (2023) PENAPISAN DAN OPTIMASI PRODUKSI LIPID KHAMIR OLEAGINOUS ASAL FERMENTASI BIJI KAKAO DENGAN VARIASI SUMBER KARBON. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Biolipid merupakan alternatif sumber energi di bumi dan dapat diperoleh dari mikroorganisme, salah satunya khamir. Khamir yang dapat menghasilkan lipid dengan 15% atau lebih dari berat kering selnya dikelompokkan ke dalam kategori oleaginous. Penelitian ini bertujuan untuk memperoleh khamir koleksi UNJCC yang termasuk dalam kategori khamir oleaginous berdasarkan penapisan secara kualitatif dengan pewarnaan Sudan III dan penapisan secara kuantitatif dengan inkubasi dalam Nitrogen Limited Medium broth, serta mengoptimasi perolehan persentase lipid khamir asal fermentasi biji kakao yaitu Rhodotorula alborubescens UNJCC Y-158, Meyerozyma guilliermondii UNJCC Y-159, Pichia kudriavzevii UNJCC Y-161 dan Pichia scutulata UNJCC Y-154 berdasarkan variasi sumber karbon meliputi jenis karbon (fruktosa, glukosa, sukrosa) dan konsentrasi karbon (30, 50, 70 g/L). Hasil deteksi kualitatif dengan pewarna Sudan III menunjukkan bahwa seluruh 11 khamir UNJCC asal fermentasi biji kakao memiliki potensi sebagai khamir oleaginous, ditandai dengan adanya warna kekuningan pada sel khamir. Berdasarkan deteksi kuantitatif diperoleh 4 dari 11 khamir UNJCC untuk uji lebih lanjut, yaitu R. alborubescens UNJCC Y-158, M. guilliermondii UNJCC Y-159, P. kudriavzevii UNJCC Y-161 dan P. scutulata UNJCC Y-154. Sementrara pada uji optimasi perolehan lipid khamir dengan variasi jenis sumber karbon dan konsentrasi karbon menunjukkan bahwa secara analisis statistik perolehan persentase lipid tertinggi diperoleh R. alborubescens UNJCC Y-158 dengan sumber karbon glukosa pada konsentrasi karbon 30 g/L senilai 18,70 ± 7,04 %, P. scutulata UNJCC Y-154 dengan sumber karbon fruktosa pada konsentrasi 30 g/L senilai 14,21 ± 3,22 %, serta P. scutulata UNJCC Y-154 dengan sumber karbon sukrosa pada konsentrasi 30 g/L senilai 16,95 ± 3,46 %. *********** Biolipids are an alternative source of energy on earth which can be derived from microorganisms, such as yeasts. Yeasts that can synthesize lipids 15% or more of their dry cell weight are called oleaginous yeasts. The study was aimed to obtain yeasts from UNJCC that are categorized as oleaginous yeast based on qualitative screening by Sudan III staining and quantitative screening by incubation in Nitrogen Limited Medium broth, to then proceed with the optimizing the selected yeasts’ lipid accumulation content namely Rhodotorula alborubescens UNJCC Y-158, Meyerozyma guilliermondii UNJCC Y-159, Pichia kudriavzevii UNJCC Y-161 and Pichia scutulata UNJCC Y-154, based on carbon source variation including carbon type (fructose, glucose, sucrose) and carbon concentration (30, 50, 70 g/L). Through applying Sudan III staining tests, it was indicated that all 11 of the UNJCC yeasts from fermented cacao beans have the potential to be oleaginous yeasts, which was supported by the presence of yellow inclusions. From the quantitative screening by incubation in Nitrogen Limited Medium, 4 yeasts, which are R. alborubescens UNJCC Y-158, M. guilliermondii UNJCC Y-159, P. kudriavzevii UNJCC Y-161 and P. scutulata UNJCC Y-154, were selected. Under variations of carbon sources type and their concentration, statistically the highest lipid concentration produced by R. alborubescens UNJCC Y-158 in 30 g/L glucose (18,70 ± 7,04%), P. scutulata UNJCC Y-154 in 30 g/L fructose (14,21 ± 3,22 %), and P. scutulata UNJCC Y-154 in 30 g/L sucrose (16,95 ± 3,46 %).

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Dalia Sukmawati, M.Si. ; 2). Ns. Sri Rahayu, S.Kep.,M.Biomed.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19499 not found.
Date Deposited:	06 Sep 2023 01:36
Last Modified:	06 Sep 2023 01:36
URI:	http://repository.unj.ac.id/id/eprint/40666

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