UJI AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK DAUN PRUMPUNG (PHRAGMITES KARKA (RETZ.) TRIN. EX STEUD)

VERDY VIRGAUTAMA, . (2023) UJI AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK DAUN PRUMPUNG (PHRAGMITES KARKA (RETZ.) TRIN. EX STEUD). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Penelitian terkait potensi antioksidan dan toksisitas ekstrak daun prumpung (Phragmites karka (Retz.) Trin. ex Steud) masih terbatas. Penelitian ini bertujuan untuk menguji aktivitas antioksidan dan toksisitas ekstrak daun prumpung dengan variasi jenis pelarut (aquades, etanol 96%, dan n-heksana) dan konsentrasi (50 ppm, 100 ppm, 150 ppm, 200 ppm, dan 250 ppm). Uji aktivitas antioksidan menggunakan metode DPPH dan ABTS. Uji toksisitas menggunakan metode BSLT. Berdasarkan hasil penelitian, ekstrak etanol 96% daun P. karka memiliki kadar total fenol dan flavonoid tertinggi, masing-masing adalah 23,97 ± 2,61 mg GAE/ mg dan 7,87 ± 1,00 mg QE/mg. Ekstrak etanol 96% daun P. karka konsentrasi 250 ppm memiliki persentase inhibisi radikal bebas DPPH dan ABTS paling signifikan, masing-masing yaitu 82,55 ± 2,03% dengan nilai IC50 sebesar 100,07 ± 0,36 ppm (sedang), dan 71,68 ± 0,91% dengan nilai IC50 sebesar 98,92 ± 1,26 ppm (kuat). Ekstrak aquades daun P. karka memiliki nilai LC50 sebesar 36,44 ppm (sangat toksik). Hasil penelitian ini diharapkan dapat memberikan informasi terkait potensi antioksidan, efek toksisitas, dan bioprospek daun prumpung. Kata kunci. ABTS, Antioksidan, BSLT, DPPH, Phragmites karka *** Research of the antioxidant potential and toxicity of Phragmites karka (Retz.) Trin. ex Steud leaf extract is still limited. This study aims to examine the antioxidant activity and toxicity of prumpung leaf extract with variations in solvents (distilled water, 96% ethanol, and n-hexane) and concentrations (50 ppm, 100 ppm, 150 ppm, 200 ppm, and 250 ppm). The antioxidant activity was tested using the DPPH and ABTS methods, while toxicity was assessed using the BSLT method. The results showed that the 96% ethanol extract of P. karka leaves had the highest total phenol and flavonoid contents, which were 23.97 ± 2.61 mg GAE/mg and 7.87 ± 1.00 mg QE/mg, respectively. The 96% ethanol extract of P. karka leaves at a concentration of 250 ppm exhibited the most significant percentage of inhibition of free radicals in DPPH and ABTS tests, which are 82,55 ± 2,03% with an IC50 value of 100,07 ± 0,36 ppm (moderate) and 71,68 ± 0,91% with an IC50 value of 98,92 ± 1,26 ppm (strong), respectively. The distilled water extract of P. karka leaves showed an LC50 value of 36,44 ppm (highly toxic). This research is expected to provide information regarding the antioxidant potential, toxicity effects, and bioprospects of prumpung leaves. Keywords. ABTS, Antioksidan, BSLT, DPPH, Phragmites karka

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. ; 2). Pinta Omas Pasaribu, M.Si.
Subjects:	Sains > Ilmu Bumi > Biologi Sains > Botani
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19562 not found.
Date Deposited:	05 Sep 2023 06:41
Last Modified:	05 Sep 2023 06:41
URI:	http://repository.unj.ac.id/id/eprint/40879

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