EFEKTIFITAS ANTIVIRAL Human Immunodeficiency Virus (HIV) TERHADAP REPLIKASI VIRUS Simian Betaretrovirus Serotype-2 (SRV-2) SECARA IN-VITRO

DINDA AISYAH FATHIA RAHMA, . (2023) EFEKTIFITAS ANTIVIRAL Human Immunodeficiency Virus (HIV) TERHADAP REPLIKASI VIRUS Simian Betaretrovirus Serotype-2 (SRV-2) SECARA IN-VITRO. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Acquired Immunodeficiency Syndrome (AIDS) merupakan penyakit yang disebabkan oleh virus Human Immunodeficiency Virus (HIV) dari keluarga Retroviridae yang dapat menyebabkan penurunan kekebalan tubuh (imunodefisiensi) pada manusia. Upaya pengobatan atau terapi terhadap HIV/AIDS menggunakan obat antiviral dilakukan untuk mencegah atau mengurangi replikasi virus tersebut. Tujuan penelitian ini adalah untuk mengetahui efektivitas obat antiviral HIV/AIDS, yaitu lopinavir, lamivudine, dan kombinasi lamivudine�zidovudine dalam menghambat replikasi virus Simian Betaretrovirus serotype-2 (SRV-2), yaitu Retrovirus yang biasa menyerang Macaca fascicularis, sehingga dapat dijadikan sebagai model dalam pengembangan obat antiviral HIV/AIDS. Parameter yang dianalisis berupa perhitungan nilai inhibitory concentration (IC50), perubahan morfologi sel, dan persentase inbhibisi replikasi SRV-2. Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL). Metode penelitian dilakukan secara kualitatif dengan mengamati perubahan morfologi sel A549 yang diinfeksikan SRV-2 (A549/SRV-2), dan secara kuantitatif dengan uji 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) untuk menghitung nilai IC50 dan uji antiviral dengan qRT-PCR untuk menentukan persentase inhibisi virus SRV-2. Data dianalisis secara kualitatif untuk perubahan morfologi sel A549/SRV-2 dan hasil uji MTT dan qRT-PCR dianalisis secara kuantitatif dengan regresi serta secara statistik dengan one-way ANOVA dan uji lanjut Duncan. Hasil pengujian sitotoksik obat antiviral terhadap sel A549 menunjukkan nilai IC50 lopinavir sebesar 74,69 ppm, lamivudine sebesar 237,92 ppm, dan lamivudine-zidovudine sebesar 188,11 ppm. Perubahan morfologi sel A549/SRV-2 menunjukkan adanya penyusutan sel dan membrane blebbing. Persentase inhibisi terhadap virus SRV-2 yang dihasilkan oleh lopinavir 100 ppm sebesar 98,36%, lamivudine-zidovudine 25 ppm sebesar 97,99%, dan lamivudine-zidovudine 100 ppm sebesar 95,12%. Obat antiviral yang efektif menghambat replikasi virus SRV-2 adalah lamivudine-zidovudine pada konsentrasi 25 ppm. **** Acquired immunodeficiency syndrome (AIDS) is a disease caused by the human immunodeficiency virus (HIV) of the Retroviridae family that causes decreased immunity (immunodeficiency) in human. Efforts to treat or therapy of HIV/AIDS using antiviral drugs are carried out to prevent or reduce the replication of the virus. The purpose of this study was to determine the effectiveness of HIV/AIDS antiviral drugs, namely lopinavir, lamivudine, and the lamivudine-zidovudine combination in inhibiting the replication of Simian Betaretrovirus serotype-2 (SRV-2) as Retrovirus that commonly infected Macaca fascicularis, therefore it could be used as a model for HIV/AIDS antiviral drug development. The parameters analyzed were based on the inhibitory concentration (IC50) values, cell morphological changes, and the percentage of SRV-2 replication inhibition. This experimental study used in this study was a completely randomized design (CRD). The research method was carried out qualitatively by observing changes in A549 infected by SRV-2 (A549/SRV-2) cell morphology, as well as quantitatively by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to measure IC50 values and antiviral test by qRT-PCR to determine percentages SRV-2 virus inhibition. Data were analyzed qualitatively for changes in cell morphology of 549/SRV-2. The results of the MTT and qRT-PCR tests were analyzed quantitatively by regression and statistically by one-way ANOVA and Duncan's further test. The results of the antiviral drug cytotoxic test on A549 cells showed an IC50 value of lopinavir of 74.69 ppm, lamivudine of 237.92 ppm, and lamivudine-zidovudine of 188.11 ppm. Changes in the morphology of A549/SRV-2 cells indicated cell shrinkage and membrane blebbing. The percentage of inhibition of the SRV-2 virus produced by 100 ppm lopinavir was 98.36%, 25 ppm lamivudine-zidovudine was 97.99%, and 100 ppm lamivudine-zidovudine was 95.12%. An antiviral drug that effectively inhibits the replication of the SRV-2 virus is lamivudine-zidovudine at a concentration of 25 ppm.

Item Type:	Thesis (Sarjana)
Additional Information:	1). drh. Atin Supiyani, M.Si. ; 2). Dr. Uus Saepuloh, S.Si., M.Biomed.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi Ilmu Kedokteran > Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan Ilmu Kedokteran > farmasi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19599 not found.
Date Deposited:	11 Sep 2023 07:09
Last Modified:	11 Sep 2023 07:09
URI:	http://repository.unj.ac.id/id/eprint/42059

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