DETEKSI GEN POLIKETIDA SINTASE TIPE II PADA AKTINOBAKTERI ASAL LAUT FLORES DAN IDENTIFIKASINYA BERDASARKAN GEN 16S rRNA

ALI AKBAR, . (2024) DETEKSI GEN POLIKETIDA SINTASE TIPE II PADA AKTINOBAKTERI ASAL LAUT FLORES DAN IDENTIFIKASINYA BERDASARKAN GEN 16S rRNA. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Aktinobakteri merupakan filum bakteri yang berperan penting sebagai agen penghasil metabolit sekunder. Salah satu metabolit sekunder yang memiliki nilai tinggi adalah poliketida. Aktinobakteri mampu mensintesis poliketida melalui jalur poliketida sintase tipe II. Tujuan penelitian untuk mengetahui karakteristik aktinobakteri asal Laut Flores, mendapatkan dan mengetahui spesies aktinobakteri asal Laut Flores yang memiliki gen pks tipe II. Metode penelitian meliputi proses isolasi aktinobakteri asal air Laut Flores dengan tiga media berbeda, amplifikasi gen pks tipe II, karakterisasi, dan identifikasi spesies aktinobakteri yang memiliki gen pks tipe II berdasarkan gen 16S rRNA. Hasil penelitian diperoleh 44 isolat aktinobakteri (5,79%) dari total 760 isolat bakteri asal Laut Flores. Sebanyak 9 isolat aktinobakteri (20,45%) menunjukkan pita dengan ukuran 600 bp. Satu isolat (MAF23-103) menunjukkan single band dan terlihat jelas. Isolat MAF23-103 memiliki sel yang berbentuk kokus dengan bentuk koloni irregular, berwarna cokelat, non-powdery, dan tidak memiliki miselia. Berdasarkan analisis sekuens 16S rRNA, isolat MAF23-103 menunjukkan similarity yang rendah yaitu sekitar 97,59% dengan Geodermatophilus nigrescens strain YIM 75980. Rendahnya persentase similarity dengan G. nigrescens strain YIM 75980 mengindikasikan kemungkinan isolat MAF23-103 merupakan spesies baru dari genus Geodermatophilus.*****Actinobacteria are a phylum of bacteria that play an important role as secondary metabolite-producing agents. One of the secondary metabolites that has high value is polyketide. Actinobacteria are able to synthesize polyketides through the Type II polyketide synthase. The purpose of the study was to determine the characteristics of actinobacteria from the Flores Sea, get and know the actinobacterial species from the Flores Sea that have type I pks genes. The research method includes the isolation process of actinobacteria from Flores Sea water with three different media, amplification of type II pks genes, characterization, and identification of actinobacterial species that have type II pks genes based on 16S rRNA genes. The results obtained 44 actinobacterial isolates (5.79%) from a total of 760 bacterial isolates from the Flores Sea. A total of 9 actinobacterial isolates (20.45%) showed a band with a size of 600 bp. One isolate (MAF23-103) showed a single band and was clearly visible. Isolate MAF23-103 has cocci-shaped cells with irregular colony shape, brown colored, non-powdery, and has no mycelia. Based on 16S rRNA sequence analysis, isolate MAF23-103 showed low similarity of about 97.59% with Geodermatophilus nigrescens strain YIM 75980. The low percentage of similarity with G. nigrescens strain YIM 75980 indicates the possibility that isolate MAF23-103 is a new species of the genus Geodermatophilus.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani, M.Si. 2). Dr. Ariani Hatmanti, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Ali Akbar .
Date Deposited:	18 Dec 2024 02:45
Last Modified:	18 Dec 2024 02:45
URI:	http://repository.unj.ac.id/id/eprint/52285

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