IDENTIFIKASI DAN UJI POTENSI BAKTERI PENDEGRADASI MIKROPLASTIK POLIPROPILENA ASAL LIMBAH PLASTIK TELUK JAKARTA (Skripsi)

SHELAVINA HAFIDZANTY, . (2025) IDENTIFIKASI DAN UJI POTENSI BAKTERI PENDEGRADASI MIKROPLASTIK POLIPROPILENA ASAL LIMBAH PLASTIK TELUK JAKARTA (Skripsi). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

	Text COVER.pdf Download (1MB)
	Text BAB I.pdf Download (170kB)
	Text BAB II.pdf Restricted to Registered users only Download (293kB) | Request a copy
	Text BAB III.pdf Restricted to Registered users only Download (329kB) | Request a copy
	Text BAB IV.pdf Restricted to Registered users only Download (541kB) | Request a copy
	Text BAB V.pdf Restricted to Registered users only Download (36kB) | Request a copy
	Text Daftar Pustaka.pdf Download (322kB)
	Text Lampiran.pdf Restricted to Registered users only Download (694kB) | Request a copy

Abstract

Pencemaran mikroplastik di Teluk Jakarta, terutama jenis polipropilena dapat menyebabkan gangguan ekologis dan fisiologis. Bioremediasi menggunakan bakteri pendegradasi mikroplastik polipropilena menjadi salah satu upaya untuk mengatasi masalah tersebut. Penelitian ini bertujuan mengetahui kemampuan bakteri pendegradasi mikroplastik polipropilena asal limbah plastik Teluk Jakarta dan identitasnya berdasarkan analisis sekuens 16S rRNA. Keenam isolat yang digunakan merupakan isolat bakteri pendegradasi mikroplastik polipropilena yang berasal dari koleksi Pusat Riset Oseanografi – BRIN. Uji degradasi selama 72 hari memperlihatkan seluruh isolat mampu mendegradasi mikroplastik polipropilena yang ditinjau berdasarkan persentase pengurangan berat kering, perubahan nilai pH media, serta struktur kimia dan fisik mikroplastik. Hasil ANOVA satu arah menunjukkan bahwa jenis bakteri berpengaruh nyata terhadap berat kering akhir mikroplastik dengan signifikansi 0,001 (P < 0,05). Persentase pengurangan berat kering terbesar adalah 7,12% (PP6). Seluruh isolat mengalami perubahan nilai pH media, peningkatan transmitansi pada gugus fungsi C – H stretching (2830 – 2955 cm-1), C – H bending (1371 – 1455 cm-1), dan C = C bending (806 – 985 cm-1), serta terbentuknya lubang pada permukaan mikroplastik. Isolat PP2 (88%), PP4 (77%), dan PP6 (82%) teridentifikasi sebagai Bacillus altitudinis berdasarkan analisis sekuens 16S rRNA. Penelitian ini memberikan informasi mengenai Bacillus altitudinis yang dapat dikembangkan sebagai agen bioremediasi. ***** Microplastic pollution in Jakarta Bay, especially polypropylene, caused ecological and physiological disturbances. Bioremediation using polypropylene microplastic degrading bacteria is one of the solutions. This study aims to determine the ability of polypropylene microplastic degrading bacteria from plastic waste in Jakarta Bay and their identity based on 16S rRNA sequence analysis. The six isolates were polypropylene microplastic degrading bacteria from the collection of Oceanographic Research Centre - BRIN. The 72-day degradation test showed that all isolates were able to degrade polypropylene microplastics based on All isolates degraded polypropylene microplastics, as indicated by reductions in dry weight, changes in pH medium, and alterations in microplastic structure. One-way ANOVA results showed that the type of bacteria had a significant effect on the final dry weight of microplastics with a significance of 0.001 (P < 0.05). The largest percentage of dry weight reduction was 7.12% (PP6). All isolates altered the pH value of the media, increased transmittance in C-H stretching (2830-2955 cm-1), C-H bending (1371-1455 cm-1), and C=C bending (806-985 cm-1), and formed surface holes in microplastics. 16S rRNA analysis identified PP2 (88%), PP4 (77%), and PP6 (82%) as Bacillus altitudinis. This study highlights Bacillus altitudinis as a potential bioremediation agent for polypropylene microplastic pollution.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani Kurniati, M. Si. 2). Milani Anggiani, M. Si.
Subjects:	Sains > Ilmu Bumi > Biologi Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 27147 not found.
Date Deposited:	03 Mar 2025 02:35
Last Modified:	03 Mar 2025 02:35
URI:	http://repository.unj.ac.id/id/eprint/53992

Actions (login required)

View Item