PRODUKSI EKSOPOLISAKARIDA OLEH ISOLAT BAKTERI ASAM LAKTAT ASAL FERMENTASI TIMUN DENGAN VARIASI JENIS DAN KONSENTRASI SUMBER KARBON

ORYZA FAUZIAH AZZAHRA, . (2025) PRODUKSI EKSOPOLISAKARIDA OLEH ISOLAT BAKTERI ASAM LAKTAT ASAL FERMENTASI TIMUN DENGAN VARIASI JENIS DAN KONSENTRASI SUMBER KARBON. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Eksopolisakarida (EPS) merupakan biopolimer karbohidrat yang disintesis dan disekresikan oleh bakteri asam laktat (BAL) secara ekstraseluler ke lingkungan sekitar selnya. Produksi EPS oleh BAL dapat dipengaruhi oleh beberapa faktor, salah satunya komposisi nutrisi yang terkandung dalam media. Penelitian ini bertujuan untuk mengidentifikasi BAL penghasil EPS asal fermentasi timun dan mengetahui pengaruh jenis dan konsentrasi sumber karbon terhadap produksi EPS, serta gugus fungsi dari EPS yang dihasilkan. Tahapan penelitian meliputi, seleksi BAL berdasarkan nilai indeks EPS, identifikasi bakteri melalui analisis gen 16S rRNA, dan produksi EPS pada variasi jenis dan konsentrasi sumber karbon, serta analisis gugus fungsi EPS dengan FTIR. Sumber karbon yang digunakan meliputi molase dan sukrosa dengan konsentrasi karbon 1%, 2%, dan 3%. Sebanyak 9 isolat BAL diseleksi pada media MRSA diperkaya susu skim 9%. Dua isolat yaitu Lactiplantibacillus argentoratensis FT1 dan isolat FT3 dipilih berdasarkan nilai indeks EPS yang dihasilkan sebesar 6,40 dan 6,89. Hasil penelitian menunjukkan isolat BAL FT3 penghasil EPS asal fermentasi timun teridentifikasi sebagai Lactiplantibacillus pentosus FT3 dengan homologi 99,16%. Berdasarkan analisis ragam tiga arah pada taraf kepercayaan 95%, jenis BAL berpengaruh nyata terhadap produksi EPS dengan nilai signifikansi < 0,05. Bakteri Lactiplantibacillus pentosus FT3 pada substrat molase 1% memproduksi EPS lebih tinggi sebesar 6,79 ± 1,22 sedangkan Lactiplantibacillus argentoratensis FT1 pada substrat molase 3% sebesar 5,58 ± 0,54. Analisis EPS dengan FTIR menunjukkan keberadaan gugus fungsional O-H (hidroksil), C-H (metil), C=O (karbonil), -COOH (karboksil) dan C-O-C (glikosidik) pada EPS. Hasil penelitian ini memberikan informasi tentang potensi isolat BAL Lactiplantibacillus argentoratensis FT1 dan Lactiplantibacillus pentosus FT3 asal fermentasi timun sebagai penghasil EPS. *****Exopolysaccharides (EPS) are carbohydrate biopolymers synthesized and secreted extracellularly by lactic acid bacteria (LAB) into their surrounding environment. The production of EPS by LAB can be influenced by several factors, one of which is the nutritional composition of the medium. This study aims to identify EPS-producing LAB from cucumber fermentation and determine the effect of different types and concentrations of carbon sources on EPS production, as well as the functional groups present in the produced EPS. The research stages included LAB selection based on the EPS index value, bacterial identification through 16S rRNA gene analysis, EPS production with variations in carbon source types and concentrations, and functional group analysis of EPS using FTIR. The carbon sources used were molasses and sucrose at carbon concentrations of 1%, 2%, and 3%. A total of nine LAB isolates were selected on MRSA medium enriched with skim milk 9%. Two isolates, namely Lactiplantibacillus argentoratensis FT1 and isolate FT3, were selected based on their EPS index values of 6.40 and 6.89, respectively. The results showed that the EPS-producing LAB FT3 isolate from cucumber fermentation was identified as Lactiplantibacillus pentosus FT3 with 99.16% homology. Based on a three-way variance analysis at a 95% confidence level, the type of LAB significantly influenced EPS production, with a significance value of <0.05. Lactiplantibacillus pentosus FT3 produced higher EPS (6.79 ± 1.22) on a 1% molasses substrate, while Lactiplantibacillus argentoratensis FT1 produced 5.58 ± 0.54 EPS on a 3% molasses substrate. FTIR analysis of EPS showed the presence of functional groups such as O-H (hydroxyl), C-H (methyl), C=O (carbonyl), -COOH (carboxyl), and C-O-C (glycosidic) in the EPS. The findings of this study provide information on the potential of Lactiplantibacillus argentoratensis FT1 and Lactiplantibacillus pentosus FT3 isolates from cucumber fermentation as EPS producers.*****

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani Kurniati, M.Si. 2). Rizky Priambodo, S.Si., M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Oryza Fauziah Azzahra .
Date Deposited:	05 Mar 2025 02:51
Last Modified:	05 Mar 2025 02:51
URI:	http://repository.unj.ac.id/id/eprint/54570

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