PENGARUH WAKTU FERMENTASI DAN RASIO LARUTAN GULA TERHADAP PRODUKSI BIOETANOL DARI GULA NIPAH (Nypa fruticans Wurmb.) OLEH Saccharomyces cerevisiae

LALA SYAFINA JIHAN, . (2025) PENGARUH WAKTU FERMENTASI DAN RASIO LARUTAN GULA TERHADAP PRODUKSI BIOETANOL DARI GULA NIPAH (Nypa fruticans Wurmb.) OLEH Saccharomyces cerevisiae. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Ketersediaan energi fosil yang semakin menipis mendorong upaya pengembangan sumber energi terbarukan, salah satunya adalah bioetanol yang dapat diproduksi dari gula nipah (Nypa fruticans Wurmb.). Penelitian ini bertujuan untuk menganalisis pengaruh kombinasi waktu dan rasio larutan gula terhadap produksi bioetanol dari gula nipah oleh Saccharomyces cerevisiae. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial dua faktor, yaitu waktu fermentasi (24; 48; dan 72 jam) dan rasio larutan gula (1:4; 1:5; 1:6). Parameter yang dianalisis meliputi perubahan kadar gula total, gula reduksi, dan pH media, serta karakteristik bioetanol yang dihasilkan, meliputi densitas, kadar, dan pH bioetanol. Berdasarkan uji ANAVA dua arah, menunjukkan bahwa kombinasi waktu fermentasi dan rasio larutan gula memberikan pengaruh nyata terhadap perubahan kadar gula total dan gula reduksi, serta kadar dan densitas bioetanol, namun tidak berpengaruh nyata terhadap perubahan pH media maupun pH bioetanol. Hasil uji lanjut DMRT menunjukkan bahwa perlakuan waktu fermentasi 48 jam dengan rasio larutan gula 1:4 (A2B1) menghasilkan kadar bioetanol tertinggi sebesar 42,97%, yang berbeda signifikan dibandingkan dengan perlakuan lainnya. Penelitian ini menunjukkan bahwa kondisi fermentasi, khususnya waktu fermentasi dan rasio larutan gula, berperan penting dalam memaksimalkan produksi bioetanol dari gula nipah. Kata kunci Bioetanol, gula nipah, rasio larutan gula, Saccharomyces cerevisiae, waktu fermentasi ***** ABSTRACT LALA SYAFINA JIHAN. The Effect of Fermentation Time and Sugar Solution Ratio on Bioethanol Production from Nipah Sugar (Nypa fruticans Wurmb.) by Saccharomyces cerevisiae. Undergraduate Thesis, Department of Biology, Faculty of Mathematics and Natural Science, Jakarta State University, July 2025. The dwindling availability of fossil energy encourages efforts to develop renewable energy sources, one of which is bioethanol which can be produced from nipah sugar (Nypa fruticans Wurmb.). This study aims to analyze the effect of the combination of time and sugar solution ratio on the production of bioethanol from nipah sugar by Saccharomyces cerevisiae. The experimental design used was a two-factor factorial completely randomized design (CRD), namely fermentation time (24; 48; and 72 hours) and sugar solution ratio (1:4; 1:5; 1:6). Parameters analyzed included changes in total sugar content, reducing sugar, and pH of the media, as well as the characteristics of bioethanol produced, including density, content, and pH of bioethanol. Based on two-way ANOVA test, the combination of fermentation time and sugar solution ratio gave a significant effect on changes in total sugar content and reducing sugar, as well as density and bioethanol content, but had no significant effect on changes in media pH or bioethanol pH. DMRT further test results showed that the treatment of 48 hours fermentation time with a sugar solution ratio of 1:4 (A2B1) produced the highest bioethanol content of 42,97%, which was significantly different compared to other treatments. This study shows that fermentation conditions, especially fermentation time and sugar solution ratio, play an important role in maximizing bioethanol production from nipah sugar. Keywords Bioethanol, nipah sugar, Saccharomyces cerevisiae, sugar solution ratio, fermentation time

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani Kurniati, M.Si. 2). Dr. Dwi Setyaningsih, S.TP., M.Si.
Subjects:	Sains > Mikro Biologi Sains > Mikro Biologi > Biokimia
Divisions:	FMIPA > S1 Biologi
Depositing User:	Lala Syafina Jihan .
Date Deposited:	15 Aug 2025 03:11
Last Modified:	15 Aug 2025 03:11
URI:	http://repository.unj.ac.id/id/eprint/61290

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