OPTIMASI DAN KARAKTERISASI FISIKOKIMIA SELULOSA BAKTERI Komagataeibacter xylinus InaCC B417 DENGAN VARIASI KONSENTRASI SUBSTRAT RUMPUT LAUT Gracilaria sp. DAN WAKTU FERMENTASI

SYARIFAH TASHARA, . (2025) OPTIMASI DAN KARAKTERISASI FISIKOKIMIA SELULOSA BAKTERI Komagataeibacter xylinus InaCC B417 DENGAN VARIASI KONSENTRASI SUBSTRAT RUMPUT LAUT Gracilaria sp. DAN WAKTU FERMENTASI. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Bakteri Komagataeibacter xylinus InaCC B417 merupakan jenis strain bakteri yang diketahui mampu mensintesis selulosa pada berbagai substrat yang mengandung nitrogen dan karbon. Rumput laut Gracilaria sp. diketahui memiliki kandungan karbon dan nitrogen yang cukup tinggi. Penelitian ini bertujuan untuk mendapatkan konsentrasi Gracilaria sp. dan waktu fermentasi optimal untuk produksi selulosa bakteri, serta mengkarakterisasi selulosa bakteri yang dihasilkan. Metode eksperimen yang digunakan pada penelitian ini yaitu metode OFAT (One Factor at Time) dengan dua faktor dan hasil penelitian dianalisis dengan Analisis Varians Satu Arah. Faktor pada penelitian ini meliputi variasi konsentrasi Gracilaria sp. (0%, 0.25%, 0.5%, 0.75%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, dan 8%) dan variasi waktu fermentasi (1-15 hari). Hasil penelitian memperlihatkan bahwa perlakuan IK2D8 (konsentrasi substrat rumput laut Gracilaria sp. 2% dan waktu fermentasi 8 hari) menghasilkan selulosa bakteri optimal dengan ketebalan 3,81 mm, berat basah 41,54 g/L, dan berat kering 2,09 g/L. Hasil karakterisasi selulosa bakteri dengan FTIR memperlihatkan adanya keberadaan gugus O-H stretching, C-H stretching, O-H bending absorbe water, C-H, C-H2, C-O-C, C-O, dan C-H out-of plane bending. Hasil SEM memperlihatkan matriks selulosa bakteri yang tersusun dari mikrofibril dengan diameter berkisar 0,139 ± 0,006 μm dan diameter porinya yaitu berkisar 0,471 ± 0,006 μm. Hasil identifikasi senyawa dengan PyGC-MS pada selulosa bakteri didapatkan 3 senyawa tertinggi yaitu L-cysteine sulfinic acid, 5-hydroxymethylfurfural, dan furfural. ***** The bacterium Komagataeibacter xylinus InaCC B417 is a type of bacterial strain known to be able to synthesize cellulose on various substrates containing nitrogen and carbon. Gracilaria sp. seaweed is known to have high carbon and nitrogen content. This study aims to obtain the optimal concentration of Gracilaria sp. and fermentation time for the production of bacterial cellulose, as well as characterize the resulting bacterial cellulose. The experimental method used in this study is the OFAT (One Factor at Time) method with two factors and the results of the study were analyzed by One-Way ANOVA. The factors in this study included variations in Gracilaria sp. concentration (0%, 0.25%, 0.5%, 0.75%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8%) and variations in fermentation time (1-15 days). The results of the study showed that IK2D8 treatment (Gracilaria sp. seaweed substrate concentration of 2% and 8 days fermentation time) produced optimal bacterial cellulose with a thickness of 3.81 mm, wet weight of 41.54 g/L, and dry weight of 2.09 g/L. The results of bacterial cellulose characterization with FTIR showed the presence of O-H stretching, C-H stretching, O-H bending absorbe water, C-H, C-H2, C-O-C, C-O, and C-H out-of plane bending groups. SEM results show that the bacterial cellulose matrix is composed of microfibrils with a diameter ranging from 0.139 ± 0.006 μm and the diameter of the pores is around 0.471 ± 0.006 μm. The results of compound identification with PyGC-MS on bacterial cellulose obtained 3 highest compounds namely L-cysteine sulfinic acid, 5-hydroxymethylfurfural, and furfural.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani Kurniati, M.Si. ; 2). Ifah Munifah, S.Pd., M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi Sains > Mikro Biologi > Biokimia Sains > Statistika
Divisions:	FMIPA > S1 Biologi
Depositing User:	Syarifah Tashara .
Date Deposited:	29 Aug 2025 07:15
Last Modified:	29 Aug 2025 07:15
URI:	http://repository.unj.ac.id/id/eprint/62254

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