RIZKY DARMANSYAH, . (2021) Uji Hepatotoksisitas Minyak Jelantah Yang Dipurifikasi Karbon Aktif Ampas Tebu Pada Tikus Putih Sprague Dawley (Rattus norvegicus). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.
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Abstract
Minyak goreng merupakan kebutuhan pokok manusia sebagai alat pengolah bahan makanan yang kebutuhannya semakin meningkat serta pemakaiannya yang berulang, sehingga menghasilkan limbah yang disebut jelantah. Perlu adanya bahan alternatif alami untuk mengurangi efek negatif minyak jelantah sehingga tidak merusak tubuh, khususnya hati, salah satu upaya adalah dengan pemanfaatan karbon aktif pada proses refineri minyak. Penelitian ini bertujuan untuk melihat hepatotoksitas minyak jelantah terhadap hati tikus.Penelitian dilakukan pada bulan Maret - Mei 2021 di Animal House FMIPA Universitas Negeri Jakarta. Metode yang digunakan adalah eksperimen dengan menggunakan jenis penelitian true eksperimental laboratorik dengan Post Test Only with Control Group Design. Sampel penelitian adalah tikus putih strain Sprague-Dawley yang dibagi dalam 4 kelompok perlakuan. Kontrol positif diberi perlakuan berupa minyak jelantah sebanyak 0,2 ml. Kontrol negatif, diberi perlakuan berupa minyak goreng kemasan sebanyak 0,2 ml. Kelompok 3 diberi perlakuan berupa minyak jelantah yang dicampur dengan karbon aktif ampas tebu sebanyak 5% dengan lama perendaman 72 jam sebanyak 0,2 ml. Kelompok 4 diberi perlakuan berupa minyak jelantah yang dicampur dengan karbon aktif ampas tebu sebanyak 15% dengan masa perendaman 72 jam sebanyak 0,2 ml. Data kadar SGPT-SGOT diukur dengan metode spektrofotometri dan dianalisis statistik dengan Anova satu arah. Hasil penelitian menunjukkan bahwa kadar SGPT dan SGOT paling tinggi terdapat pada kelompok perlakuan minyak jelantah yaitu 29,59±1,417 U/L dan 26,10±0,408 U/L, diikuti dengan kelompok perlakuan minyak jelantah yang dicampur dengan karbon aktif ampas tebu sebanyak 15% yaitu 24,7±0,627 U/L dan 24,7±0,627 U/L, kemudian kelompok perlakuan minyak jelantah yang dicampur dengan karbon aktif ampas tebu sebanyak 5% yaitu 18,85±0,544 U/L dan 18,85±0,544 U/L, lalu kelompok perlakuan minyak goreng yaitu 17,37±0,93 U/L dan 17,37±0,93 U/L. Perlakuan minyak jelantah dengan karbon aktif ampas tebu sebanyak 5% memiliki potensi sebagai adsorben untuk purifikasi minyak jelantah. Cooking oil is a basic human need as a food processing tool whose needs are increasing and its use is repeated, resulting in waste called waste cooking. There is a need for natural alternative materials to reduce the negative effects of used cooking oil so that it does not damage the body, especially the liver, one of the efforts is the use of activated carbon in the oil refinery process. This study aims to determine the hepatotoxicity of used cooking oil on the liver of rats. The study was conducted from March 2021 to May 2021 at the Animal House FMIPA, State University of Jakarta. The method used is an experiment using a true experimental laboratory type of research with Post Test Only with Control Group Design. The research sample was Sprague-Dawley strain white rats which were divided into 4 treatment groups and 5 replications. Positive control was given treatment in the form of cooking oil as much as 0.2 ml. Negative control, given treatment in the form of packaged cooking oil as much as 0.2 ml. Group 3 was treated in the form of used cooking oil mixed with 5% of sugarcane bagasse activated carbon with a 72 hour soaking time of 0.2 ml. Group 4 was treated in the form of used cooking oil mixed with 15% sugarcane bagasse activated carbon with a 72 hour soaking period of 0.2 ml. Data on SGPT-SGOT levels were measured by spectrophotometric method and statistically analyzed with one-way ANOVA. The results showed that the highest levels of SGPT and SGOT were found in the used cooking oil treatment group, namely 29.59±1.417 U/L and 26.10±0.408 U/L, followed by the used cooking oil treatment group mixed with bagasse activated carbon as much as 15 %, namely 24.7±0.627 U/L and 24.7±0.627 U/L, then the treatment group of used cooking oil mixed with bagasse activated carbon was 5%, namely 18.85±0.544 U/L and 18.85±0.544 U/L, then the cooking oil treatment group were 17.37±0.93 U/L and 17.37±0.93 U/L. The treatment of used cooking oil with 5% of sugarcane bagasse activated carbon has potential as an adsorbent for purification of used cooking oil.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Additional Information: | 1) Ns. Sri Rahayu, M.Biomed. 2)drh. Atin Supiyani, M.Si. |
| Subjects: | Sains > Ilmu Bumi > Biologi |
| Divisions: | FMIPA > S1 Biologi |
| Depositing User: | Users 14629 not found. |
| Date Deposited: | 13 Jul 2022 02:54 |
| Last Modified: | 13 Jul 2022 02:54 |
| URI: | http://repository.unj.ac.id/id/eprint/31688 |
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