IDENTIFIKASI POLIMORFISME GEN NCAPG (c.1326T>G) PADA SAPI LIMOUSIN JANTAN (Bos taurus) MENGGUNAKAN METODE PCR-RFLP

LILIA SISWARDANI, . (2022) IDENTIFIKASI POLIMORFISME GEN NCAPG (c.1326T>G) PADA SAPI LIMOUSIN JANTAN (Bos taurus) MENGGUNAKAN METODE PCR-RFLP. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

*ABSTRAK* Gen non-SMC condensin I complex subunit G (NCAPG) merupakan salah satu gen kunci yang mempengaruhi perkembangan otot. Polimorfisme c.1326T>G pada gen NCAPG berpotensi sebagai kandidat marker sifat karkas pada sapi. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme gen NCAPG c.1326T>G pada sapi Limousin jantan. Penelitian ini menggunakan sampel semen beku dari 100 ekor sapi pejantan Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan Singosari (BBIB Singosari) dan Balai Inseminasi Buatan Lembang (BIB Lembang). Isolasi DNA dilakukan menggunakan gSYNCTM DNA extraction kit. Genotyping dilakukan menggunakan metode PCR-RFLP dengan enzim restriksi TasI. Parameter penelitian meliputi frekuensi genotipe dan alel, keseimbangan Hardy-Weinberg (HWE), nilai heterozigositas pengamatan (Ho), dan heterozigositas harapan (He). Hasil penelitian menunjukkan SNP c.1326T>G gen NCAPG pada populasi sapi Limousin jantan bersifat polimorfisme dengan memiliki keragaman yang rendah dibuktikan teridentifikasi tiga genotipe (TT, TG dan GG) dengan frekuensi berturut-turut sebesar 0,520; 0,390 dan 0,090 serta dua alel (T dan G) dengan frekuensi berturut-turut sebesar 0,715 dan 0,285. Nilai Ho dan He berturut-turut sebesar 0,390 dan 0,408, yang menunjukkan bahwa SNP c.1326T>G gen NCAPG pada populasi sapi Limousin jantan yang diteliti memiliki keragaman yang rendah. Berdasarkan hasil tersebut disimpulkan bahwa SNP c.1326T>G gen NCAPG pada sapi Limousin jantan bersifat polimorfik dengan keragaman yang rendah. Hasil penelitian ini masih perlu dilanjutkan pada analisis asosiasi antara SNP c.1326T>G gen NCAPG dengan sifat karkas untuk menentukan apakah dapat digunakan sebagai marker genetik dalam program pemuliaan sapi pada sifat karkas. Kata kunci: gen NCAPG, karkas sapi, PCR-RFLP, polimorfisme, SNP c.1326T>G *ABSTRACT* The non-SMC condensin I complex subunit G (NCAPG) gene is one of the key genes regulating muscle development. The c.1326T>G polymorphism in NCAPG gene has the potential as a candidate marker for carcass traits in beef cattle. The aim of this study was to identify the polymorphism of c.1326T>G NCAPG gene in Limousin bulls. The study used frozen semen samples from 100 Limousin bulls produced by the National Artificial Insemination of Singosari (BBIB Singosari) and Lembang (BIB Lembang). DNA isolation was performed using the gSYNCTM DNA extraction kit. Genotyping was carried out using the PCR-RFLP method with the TasI restriction enzyme. The parameters of this study included genotype and allele frequencies, Hardy-Weinberg equilibrium (HWE), observed (Ho) and expected heterozygosity (He). The results showed that three genotypes (TT, TG, and GG) had been identified with the frequencies of 0,520; 0,390 and 0,090, and two alleles (T and G) with the frequencies of 0,715 and 0,285. The Ho and He values were 0,390 and 0,408, indicating that the SNP c.1326T>G NCAPG gene in the population of Limousin bulls studied had low polymorphism. Based on these results, it was concluded that the SNP of c.1326T>G NCAPG gene in Limousin bulls was polymorphic with low polymorphism. The results of this study, still need to be further investigated in the association analysis between the SNP of c.1326T>G NCAPG gene with carcass traits to determine whether it can be used as a genetic marker or not in beef cattle breeding programs on carcass traits. Keywords: beef carcass, NCAPG gene, PCR-RFLP, polymorphism, SNP c.1326T>G

Item Type:	Thesis (Sarjana)
Additional Information:	1) Dr. Yulia Irnidayanti, M.Si 2) Saiful Anwar, M.Si
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Ilmu Bumi > Biologi Sains > Ilmu Bumi > Genetika Sains > Zoologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 15746 not found.
Date Deposited:	05 Sep 2022 02:56
Last Modified:	05 Sep 2022 02:56
URI:	http://repository.unj.ac.id/id/eprint/34629

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