MORFOMETRI MUKOSA DUODENUM DAN JUMLAH SEL POLIMORFONUKLEAR MENCIT HIPERKOLESTEROLEMIA YANG DIBERI SUSU SINBIOTIK DAN MINYAK TRANS

INDAH AGUSSETIANDARI, . (2022) MORFOMETRI MUKOSA DUODENUM DAN JUMLAH SEL POLIMORFONUKLEAR MENCIT HIPERKOLESTEROLEMIA YANG DIBERI SUSU SINBIOTIK DAN MINYAK TRANS. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

*ABSTRAK* Hiperkolesterolemia dapat memicu stress oksidatif dan mengaktivasi sel radang (sel polimorfonuklear/PMN). Susu sinbiotik merupakan susu hasil fermentasi probiotik (Lactobacillus acidophilus) dikombinasikan dengan prebiotik seperti tepung pisang tanduk (TP). Penelitian ini bertujuan mengetahui pengaruh pemberian susu sinbiotik (SS) terhadap struktur mukosa duodenum dan jumlah sel polimorfonuklear mencit (Mus musculus) hiperkolesterolemia yang diberi minyak trans (MT). Metode penelitian yaitu eksperimental dengan desain Rancangan Acak Lengkap. Sebanyak 30 ekor mencit jantan strain DDY berumur 2 bulan dibagi menjadi 6 kelompok yaitu Kontrol negatif (MT); Kontrol positif (MT+simvastatin dosis 0,4 ml/20grBB); MT+SF 0% TP; MT+SS 1% TP; MT+SS 3% TP; dan MT+SS 5% TP. Dosis MT sebesar 200mg/KgBB/hari dan SS sebesar 200mg/20grBB/hari. Morfologi mukosa duodenum dianalisis secara deskriptif. Data morfometri duodenum berupa tinggi vili, tebal mukosa, dan jumlah sel polimorfonuklear dianalisis secara statistik dengan oneway ANOVA (P<0,05). Hasil menunjukkan bahwa pemberian susu sinbiotik tidak signifikan terhadap tebal mukosa, tinggi vili, dan jumlah sel polimorfonuklear mukosa duodenum (P>0,05). Namun, ditemukan erosi vili dan deskuamasi epitel mukosa duodenum pada kelompok positif dan negatif. Sementara pada kelompok susu sinbiotik semakin tinggi persentase tepung pisang kondisi erosi vili dan deskuamasi epitel mukosa duodenum semakin sedikit. Kelompok susu sinbiotik 5% prebiotik tidak terdapat erosi vili dan deskuamasi epitel duodenum. Maka dapat disimpulkan bahwa pemberian susu sinbiotik tidak memengaruhi tebal mukosa, tinggi vili, dan jumlah sel polimorfonuklear, namun memperbaiki struktur mukosa duodenum dari erosi vili dan deskuamasi epitel akibat minyak trans. Susu sinbiotik dapat menjadi pangan olahan yang dapat meningkatkan kualitas sistem pencernaan. Kata kunci: Minyak trans, Mukosa duodenum, Sel Polimorfonuklear, dan Susu sinbiotik. *ABSTRACT* Hypercholesterolemia can trigger oxidative stress and activate cells including (polymorphonuclear cells/PMN). Synbiotic milk is probiotic fermented milk (Lactobacillus acidophilus) combined with prebiotics such as tanduk banana flour (TP). This study aims to determine the effect of giving synbiotic milk (SS) on the structure of the duodenal mucosa and the number of polymorphonuclear cells of hypercholesterolemic mice (Mus musculus) given trans oil (MT). The research method is the Completely Randomized Design. A total of 30 male mice with the DDY strains aged 2 months were divided into 6 groups, namely negative control (MT); Positive control (MT+simvastatin dose 0,4 ml/20grBB); MT+SF 0% TP; MT+SS 1% TP; MT+SS 3% TP; and MT+SS 5% TP. The MT dose is 200mg/KgBB/day and the SS is 200mg/20grBB/day. Duodenal mucosal morphology was analyzed descriptively. Duodenal morphometry data in the form of villi height, mucosal thickness, and number of polymorphonuclear cells were statistically analyzed by one-way ANOVA (P<0,05). The results showed that the administration of synbiotic milk had no significant effect on mucosal thickness, villi height, and the number of polymorphonuclear cells of the duodenal mucosa (P>0,05). However, villous erosion and desquamation of the duodenal mucosal epithelium were in the positive and negative groups. Meanwhile, in the SS group, the higher the percentage of TP, the condition of villous erosion and desquamation of the duodenal mucosal epithelium decreased. The prebiotic 5% SS group did not have villous erosion and duodenal epithelial desquamation. So it can be said that presenting SS did not affect the morphometry of mucosal thickness, villi height, and polymorphonuclear cell count, but improved duodenal mucosa from villous erosion and epithelial desquamation due to trans oil. Synbiotic milk can be processed food that can improve the quality of the digestive system. Keywords: Mucosa, Polymorphonuclear Cells, Synbiotic Milk, and Trans Oil.

Item Type:	Thesis (Sarjana)
Additional Information:	1) drh. Atin Supiyani, M.Si 2) Dr. Tri Handayani Kurniati, M.Si
Subjects:	Sains > Ilmu Bumi > Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 15743 not found.
Date Deposited:	06 Sep 2022 02:30
Last Modified:	06 Sep 2022 02:30
URI:	http://repository.unj.ac.id/id/eprint/35002

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