KEMAMPUAN ANTIHEMOLISIS EKSTRAK DAUN KALIANDRA (Calliandra calothyrsus) TERHADAP MENCIT YANG DIINDUKSI MINYAK TRANS

KHOLISAH KHANSA, . (2023) KEMAMPUAN ANTIHEMOLISIS EKSTRAK DAUN KALIANDRA (Calliandra calothyrsus) TERHADAP MENCIT YANG DIINDUKSI MINYAK TRANS. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Eritrosit merupakan jenis sel darah yang memiliki peran penting dalam mengangkut oksigen dalam tubuh. Namun, konsumsi minyak trans dapat meningkatkan produksi radikal bebas yang berpotensi merusak membran sel eritrosit atau hemolisis dan menimbulkan berbagai masalah kesehatan. Ekstrak daun kaliandra yang mengandung senyawa metabolit sekunder seperti flavonoid sebagai antioksidan untuk melindungi membran sel eritrosit dari kerusakan akibat radikal bebas. Tujuan penelitian ini untuk mengukur kemampuan antihemolisis ekstrak daun kaliandra pada mencit yang diinduksi minyak trans ditinjau dari persentase inhibisi hemolisis, jumlah eritrosit, kadar hemoglobin dan indeks organ hati. Metode penelitian eksperimental RAL post test design serta analisis data menggunakan uji one-way ANOVA. Mencit Jantan strain DDY sebanyak 25 ekor berumur 2-3 bulan dibagi menjadi 5 kelompok yaitu kelompok negatif (minyak trans); kelompok plasebo (aquades); perlakuan minyak trans 200 mg/kgBB + (ekstrak daun kaliandra 2, 4 dan 6 mg/kgBB) yang diinduksi selama 30 hari. Hasil penelitian ini menunjukkan bahwa ekstrak daun kaliandra memiliki kemampuan antihemolisis terhadap mencit yang diinduksi minyak trans pada dosis 2 mg/kgBB yang ditinjau dari berupa persentase inhibisi hemolisis sebesar 43,9-77,8%; jumlah eritrosit sebesar 6,6-9,1 x 106 sel/μL; jumlah hemoglobin sebesar 12,3-15,6 g/dL. Ekstrak daun kaliandra efektif dalam mempertahankan jumlah eritrosit normal pada mencit yang diinduksi minyak trans. Penelitian ini dapat menambah informasi dan membuka peluang baru potensi ekstrak daun kaliandra sebagai agen antihemolisis untuk pengembangan obat berbagai penyakit **************** Erythrocytes are type of blood cell with a crucial role in transporting oxygen throughout the body. However, the consumption of trans fats can elevate the production of free radicals, potentially damaging erythrocyte cell membranes, leading to hemolysis, and causing various health issues. The extract of Caliandra leaves, containing secondary metabolites such as flavonoids, acts as an antioxidant to protect erythrocyte cell membranes from damage caused by free radicals. This study aims to assess the antihemolytic capability of Caliandra leaf extract in mice induced with trans fats, based on parameters including the percentage of hemolysis inhibition, erythrocyte count, hemoglobin levels, and liver organ index. An experimental research design using a Randomized Block Design (RAL) with a post-test approach was employed, and data analysis utilized one-way ANOVA. A total of 25 male DDY strain mice aged 2-3 months were divided into 5 groups: the negative control group (trans fat only), the placebo control group (aquadest), and three treatment groups (trans fat 200 mg/kgBB + Caliandra leaf extract 2, 4, and 6 mg/kgBB) induced for 30 days. Results from this study demonstrate that Kaliandra leaf extract possesses antihemolytic properties in trans fat-induced mice at a dosage of 2 mg/kgBW, as indicated by a hemolysis inhibition percentage ranging from 43,9% to 77,8%, erythrocyte counts of 6,6 to 9,1 x 106 cells/μL, and hemoglobin levels of 12,3 to 15,6 g/dL. Caliandra leaf extract effectively maintains normal erythrocyte counts in trans fat-induced mice. This research provides additional insights and opens new possibilities for the potential use of Kaliandra leaf extract as an antihemolytic agent in the development of drugs for various diseases.

Item Type:	Thesis (Sarjana)
Additional Information:	1). drh. Atin Supiyani, M.Si. ; 2). Drs. Refirman Dj, M.Biomed.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Fisiologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 19402 not found.
Date Deposited:	05 Sep 2023 02:40
Last Modified:	05 Sep 2023 02:40
URI:	http://repository.unj.ac.id/id/eprint/40550

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