Potensi Antiseptik Ekstrak Kapang Endofit Daun Mangrove (Rhizophora sp.) terhadap Penghambatan Kapang Patogen UNJCC F-132 Fusarium oxysporum Asal Saliva Caplak Ular Sanca (Malayophyton reticulatus)

FAMIRA ICHSANTY, . (2024) Potensi Antiseptik Ekstrak Kapang Endofit Daun Mangrove (Rhizophora sp.) terhadap Penghambatan Kapang Patogen UNJCC F-132 Fusarium oxysporum Asal Saliva Caplak Ular Sanca (Malayophyton reticulatus). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Ekstrak kapang endofit asal daun mangrove (Rhizophora sp.) memiliki senyawa yang bersifat sebagai antikapang, sehingga dapat dijadikan inovasi sebagai zat aktif ke dalam antiseptik untuk upaya menghambat pertumbuhan kapang patogen UNJCC F-132 F. oxysporum asal saliva caplak ular sanca (M. reticulatus). Penelitian ini bertujuan melakukan identifikasi kapang endofit daun mangrove, mendapatkan ekstrak kapang endofit daun mangrove yang bersifat tidak toksik, mendapatkan senyawa bioaktif kapang endofit daun mangrove melalui analisis GC-MS, dan melakukan pengujian antagonis antiseptik ekstrak kapang endofit daun mangrove terhadap penghambatan kapang patogen UNJCC F-132 F. oxysporum. Metode penelitian yang dilakukan adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL). Identifikasi kapang endofit dilakukan pada daerah ITS4 dan ITS5 ribosomal DNA dan karakterisasi morfologi. Pengujian pada penelitian ini berupa uji senyawa volatil, uji toksisitas, dan uji antagonis sediaan antiseptik. Hasil identifikasi molekular pada dua kapang endofit potensial menunjukkan kedua kapang endofit tersebut memiliki jenis Fusarium solani. Persentase penghambatan terhadap UNJCC F-132 Fusarium oxysporum pada uji volatil dikategorikan sebagai penghambatan sedang dengan dua isolat kapang endofit memiliki persentase UNJCC F115 (66,52%) dan UNJCC F116 (55,32%). Hasil fermentasi dari kedua kapang endofit bersifat tidak toksik terhadap A. salina dengan LC50 > 1000 ppm. Analisis GC-MS didapatkan 5 golongan senyawa dengan total 12 jenis senyawa yang terdeteksi sebagai alkohol (4 jenis), asam (1 jenis), alkaloid (1 jenis), fenol (2 jenis), dan ester (1 jenis). Hasil pengujian antagonis sediaan antiseptik ekstrak kapang endofit tidak berpengaruh nyata terhadap persentase penghambatan kapang patogen dengan persentase UNJCC F115 F. solani (13,82%-36,36%) dan UNJCC F116 F. solani (11,46%-21,08%). Extract of endophytic fungi from mangrove leaves (Rhizophorasp.) have compounds that act as antifungi, so it can be used as an innovation as an active substance in an antiseptic to inhibit the growth of the pathogenic fungus UNJCC F-132 F. oxysporum from the saliva of python ticks (M. reticulatus). This research aims to identify mangrove leaf endophytic fungi, obtain non-toxic extracts of mangrove leaf endophytic fungi, obtain bioactive compounds of mangrove leaf endophytic fungi through GC-MS analysis, and test antiseptic antagonists of mangrove leaf endophytic fungi extracts against the inhibition of pathogenic fungi UNJCC F-132 F. oxysporum. The research method used was experimental using a Completely Randomized Design (CRD). Identification of endophytic fungi was carried out in the ITS4 and ITS5 regions of ribosomal DNA and morphological characterization. The tests in this research consisted of volatile compound tests, toxicity tests, and antagonist tests for antiseptic preparations. The results of molecular identification on two potential endophytic fungi show that both endophytic fungi are of the Fusarium solani type. The percentage of inhibition against UNJCC F-132 Fusarium oxysporum in the volatile test was categorized as moderate inhibition with two endophytic fungi isolates having a percentage of UNJCC F115 (66.52%) and UNJCC F116 (55.32%). The fermentation results from both endophytic fungi are non-toxic to A. salina with LC50 > 1000 ppm. GC-MS analysis revealed 5 groups of compounds with a total of 12 types of compounds detected as alcohols (4 types), acids (1 type), alkaloids (1 type), phenols (2 types), and esters (1 type). The results of antagonist testing of antiseptic preparations from endophytic fungi extracts had no significant effect on the percentage of inhibition of pathogenic fungi with the percentages of UNJCC F115 F. solani (13.82%-36.36%) and UNJCC F116 F. solani (11.46%-21.08% ).

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Dalia Sukmawati, M.Si. ; 2). Mohammad Isnin Noer, M.Si
Subjects:	Sains > Kimia Sains > Mikro Biologi > Biokimia
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 24209 not found.
Date Deposited:	07 Aug 2024 01:19
Last Modified:	07 Aug 2024 01:19
URI:	http://repository.unj.ac.id/id/eprint/48868

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