EKSTRAKSI DAN KARAKTERISASI KANDUNGAN FENOLIK KULIT BUAH RAMBUTAN VARIETAS PARAKAN (Nephelium lappaceum. Linn) SERTA UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH

AULADINA YASYFA, . (2022) EKSTRAKSI DAN KARAKTERISASI KANDUNGAN FENOLIK KULIT BUAH RAMBUTAN VARIETAS PARAKAN (Nephelium lappaceum. Linn) SERTA UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Salah satu varietas Rambutan khas Tangerang, Banten yaitu Rambutan (Nephelium lappaceum. Linn) varietas Parakan dimanfaatkan untuk penelitian ini karena belum ada publikasinya. Pada penelitian ini, diidentifikasi komponen fenolik serta menentukan kadar total fenolik dan menguji aktivitas antioksidan dari kulit rambutan varietas parakan. Sebanyak 200 gram serbuk kering kulit buah rambutan varietas parakan dimaserasi secara bertahap dengan menggunakan pelarut n-heksana, etil asetat dan metanol. Hasil rendemen dari ekstrak metanol dan ekstrak etil asetat yaitu 36,88% dan 8,74%. Hasil uji fitokimia dalam kulit rambutan parakan positif mengandung fenolik, alkaloid, flavonoid dan steroid. Identifikasi golongan fenolik dilakukan dengan Kromatografi Lapis Tipis (KLT) dan Gas Chromatography-Mass Spectrometry (GC-MS), sehingga hasil dari GCMS diperoleh beberapa senyawa fenolik dominan yaitu katekol (m/z 110 g.mol-1) dan 1,2,3-benzentriol (m/z 126 g.mol-1) dari ektrak etil asetat dan ekstrak metanol. Hasil uji kadar total fenolik dari ekstrak metanol diperoleh 34,4%. Kemudian ekstrak metanol dan etil asetat diuji aktivitas antioksidan dengan menggunakan metode 1,1-difenil-2-pikrilhidrazil (DPPH) yang dinyatakan dengan nilai Inhibition Concentration 50% (IC50) masing-masing yaitu 8,230 mg/L dan 9,904 mg/L. Kesimpulan dari penelitian ini yaitu kulit rambutan mengandung komponen fenolik yang relatif tinggi dengan kategori aktivitas antioksidan yang sangat aktif. ************************************* One of the typical Rambutan varieties of Tangerang, Banten namely Rambutan (Nephelium lappaceum. Linn) Parakan variety was used for this research because it has not yet been published. In this study, identified phenolic components, determined total phenolic content and tested the antioxidant activity of rambutan peels of parakan variety. A total of 200 grams of dry powder of rambutan peel of parakan variety was macerated gradually using n-hexane, ethyl acetate and methanol as solvents. The yields of methanol extract and ethyl acetate extract were 36,88% and 8,74%, respectively. Phytochemical test results in parakan rambutan peel positive contain phenolic, alkaloids, flavonoids and steroids. Identification of phenolic groups was carried out by Thin Layer Chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GC-MS), so that the results of GC-MS obtained several dominant phenolic compounds, namely catechol (m/z 110 g.mol-1) and 1,2,3-benzentriol (m/z 126 g.mol-1) from ethyl acetate extract and methanol extract. The results of the total phenolic content of the methanol extract were 34,4%. Then the methanol and ethyl acetate extracts were tested for antioxidant activity using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method which was expressed with IC50 values of 8,230 mg/L 9,904 mg/L, respectively. The conclusion of this study is that rambutan peel contains relatively high phenolic components with a very active category of antioxidant activity.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Zulhipri, M.Si.; 2). Dr. Hanhan Dianhar, M.Si.
Subjects:	Sains > Kimia
Divisions:	FMIPA > S1 Kimia
Depositing User:	sawung yudo
Date Deposited:	29 Oct 2024 04:00
Last Modified:	29 Oct 2024 04:00
URI:	http://repository.unj.ac.id/id/eprint/51794

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