FIRDA KAMILA, . (2022) PROFIL HEMATOLOGIS DAN KADAR ANTIOKSIDAN ENDOGEN DARAH Rattus norvegicus YANG DIBERI EKSTRAK DAUN SIMPOR (Dillenia suffruticosa Martelli.). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.
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Abstract
Stres oksidatif adalah kondisi yang terjadi akibat adanya produksi radikal bebas berlebih dalam tubuh. Salah satu penyebab stres oksidatif yaitu konsumsi minyak goreng dengan pemanasan tinggi (minyak trans). Oleh karena itu, tubuh memerlukan antioksidan yang mampu menetralkan radikal bebas dalam tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun simpor terhadap profil hematologis dan kadar enzim antioksidan endogen superoksida dismutase (SOD) dan glutation peroksidase (GPX) darah tikus yang mengalami stres oksidatif. Penelitian dilakukan pada bulan November 2020-September 2021. Metode penelitian yang digunakan yaitu metode eksperimental dengan desain Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan 25 sampel darah tikus yang dibagi menjadi 5 kelompok perlakuan yang terdiri dari kelompok tanpa perlakuan (kontrol positif), kelompok dengan pemberian minyak trans (kontrol negatif), kelompok dengan pemberian ekstrak daun simpor 500 mg/kg BB, 1000 mg/kg BB dan 2000 mg/kg BB selama 7 hari. Pengukuran kadar antioksidan endogen dengan metode spektofotometri menggunakan kit Randox®. Profil hematologis diukur menggunakan hematology analyzer. Analisis data dilakukan dengan uji One Way ANOVA pada α=0,05 dan uji lanjut Duncan. Hasil penelitian menunjukkan kadar hemoglobin tikus yang diberi ekstrak daun simpor dosis 500 mg/kg BB berbeda nyata dengan kontrol negatif (α<0,05). Jumlah eritrosit, kadar SOD dan GPX yang diberi ekstrak daun simpor tidak berbeda nyata dari kontrol negatif (α>0,05). Dapat disimpulkan, ekstrak daun simpor dalam penelitian ini tidak berpengaruh terhadap jumlah eritrosit serta kadar enzim antioksidan endogen SOD dan GPX, tetapi berpengaruh terhadap kadar hemoglobin. ********************************************************** Oxidative stress is a condition that occurs due to the production of excess free radicals in the body. One of the causes of oxidative stress is the consumption of cooking oil with high heating (trans oil). Therefore, the body needs antioxidants that can neutralize free radicals in the body. This study aims to determine the effect of simpor leaf extract on the hematological profile and levels of endogenous antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the blood of rats subjected to oxidative stress. The study was conducted in November 2020-September 2021. The research method used was an experimental method with a Completely Randomized Design (CRD). This study used 25 rat blood samples which were divided into 5 treatment groups consisting of a group without treatment (positive control), a group given trans oil (negative control), a group given simpor leaf extract 500 mg/kg BW, 1000 mg/kg. BW and 2000 mg/kg BW for 7 days. Measurement of endogenous antioxidant levels using the spectrophotometric method using the Randox® kit. Hematological profile was measured using a hematology analyzer. Data analysis was performed using One Way ANOVA test at α =0.05 and Duncan's follow-up test. The results showed that the hemoglobin levels of rats given simpor leaf extract at a dose of 500 mg/kg BW were significantly different from that of the negative control (α<0.05). The number of erythrocytes, levels of SOD and GPX given simpor leaf extract was not significantly different from the negative control (α> 0.05). It can be concluded, simpor leaf extract in this study had no effect on the number of erythrocytes and levels of endogenous antioxidant enzymes SOD and GPX, but did affect hemoglobin levels.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Additional Information: | 1). Ns. Sri Rahayu M.Biomed.; 2). drh. Atin Supiyani, M.Si |
| Subjects: | Sains > Ilmu Bumi > Biologi |
| Divisions: | FMIPA > S1 Biologi |
| Depositing User: | sawung yudo |
| Date Deposited: | 05 Nov 2024 04:10 |
| Last Modified: | 05 Nov 2024 04:10 |
| URI: | http://repository.unj.ac.id/id/eprint/51918 |
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