MARLINA AZZAHRA, . (2022) ANALISIS SITOSTATIK DAN GENOTOKSISITAS RADIASI GAMMA PADA LIMFOSIT DARAH PEKERJA BERDASARKAN GOLONGAN DARAH DAN USIA: UJI CBMN. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.
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Abstract
Radiasi sinar gamma menyebabkan genotoksisitas, yang ditandai dengan pembentukan badan inti ekstra. Ini berisi kromosom yang rusak, tertinggal pada anafase selama pembelahan sel. Badan ekstranuklear dapat disebut mikronukleus (MN), yang menunjukkan bahwa radiasi gamma bersifat genotoksik. Radiasi juga dapat menyebabkan gangguan pembelahan sel dan dapat digunakan sebagai biomarker untuk memprediksi tingkat risiko terhadap kesehatan manusia. Penelitian ini bertujuan untuk menganalisis efek genotoksik dan sitotoksik radiasi gamma terhadap limfosit pekerja di pusat energi radiasi menggunakan CBNM Assay. Sampel darah dari 20 sukarelawan dikumpulkan dalam tabung heparin dan dibiakkan secara in vitro. Hasil penelitian menunjukkan bahwa gamma 2 Gy menyebabkan peningkatan jumlah mikronukleus limfosit darah terutama pada golongan darah A dan peningkatan jumlah MN tidak berhubungan dengan usia. Selain itu, nilai NDI pada limfosit darah manusia setelah radiasi gamma adalah 1,74 ± 0,1. Nilai tersebut masih dalam kategori normal dan tidak terlalu berbahaya bagi kesehatan manusia. Dapat disimpulkan bahwa iradiasi gamma tidak memiliki efek sitostatik tetapi memiliki efek genotoksik pada limfosit darah in vitro. *********************************************************** Gamma-ray radiation causes genotoxicity, which is characterized by the formation of extra-nuclear bodies. It contains defective chromosomes, left behind at anaphase during cell division. The extranuclear body can be called a micronucleus (MN), which indicates that gamma radiation is genotoxic. Radiation can also be caused by interference of cell division and can be used as a biomarker to predict the level of risk to human health. This study aims to analyze the genotoxic and cytotoxic effects of gamma radiation on worker lymphocytes in radiation energy centers using the CBNM Assay. Blood samples from 20 volunteers were collected in heparin tubes and cultured in vitro. The results showed that gamma 2 Gy caused an increase in the number of micronuclei of blood lymphocytes, especially in blood type A and the increase in the number of MN was not related to age. In addition, the NDI value of lymphocytes in human blood after gamma radiation is 1.74 ± 0.1. This value was still in the normal category and not too harmful for human health. It can be concluded that gamma irradiation has no cytostatic effects but has a genotoxic effect on blood lymphocytes in vitro.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Additional Information: | 1). Dr. Yulia Irnidayanti, M.Si.; 2). Dra. Yanti Lusiyanti. |
| Subjects: | Sains > Ilmu Bumi > Biologi |
| Divisions: | FMIPA > S1 Biologi |
| Depositing User: | sawung yudo |
| Date Deposited: | 08 Nov 2024 08:46 |
| Last Modified: | 08 Nov 2024 08:46 |
| URI: | http://repository.unj.ac.id/id/eprint/52036 |
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