EFEK KOMBINASI RESVERATROL DAN DOXORUBICIN TERHADAP SITOTOKSISITAS, RESPON SPHEROID DAN MIGRASI SEL SH-SY5Y

SHAFIRA ALIFIA RAMADHAN, . (2025) EFEK KOMBINASI RESVERATROL DAN DOXORUBICIN TERHADAP SITOTOKSISITAS, RESPON SPHEROID DAN MIGRASI SEL SH-SY5Y. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Doxorubicin telah menjadi obat kemoterapi utama kanker sejak lama. Obat ini memiliki efek samping yang cukup serius ketika diberikan secara terus-menerus seperti neurotoksisitas melalui stres oksidatif pada sel saraf normal dan neuropati perifer dikarenakan jenis kematian selnya tidak sepenuhnya berupa kematian sel terprogram (apoptosis). Solusi efek samping tersebut adalah dengan mengkombinasikan doxorubicin dengan senyawa antikanker berkadar toksik rendah terhadap sel normal dan senyawa yang dominan mengarahkan kematian sel kanker pada kematian sel terprogram seperti resveratrol. Penelitian ini bertujuan untuk mengetahui potensi resveratrol sebagai agen adjuvan kemoterapi terhadap doxorubicin melalui kadar sitotoksisitas, sinergisitas senyawa, migrasi sel, dan respon spheroid terhadap sel kanker Neuroblastoma SH-SY5Y. Kelompok perlakuan dibagi menjadi kelompok doxorubicin 0,005 µM, resveratrol 40 µM, resveratrol 60 µM, kombinasi doxo+res 40 µM, kombinasi doxo+res 60 µM, dan kontrol. Hasil penelitian menunjukkan kombinasi doxorubicin dan resveratrol secara sinergis mematikan sel SH-SY5Y, menghambat migrasi sel, dan menghambat proliferasi serta metastasis sel SH-SY5Y dalam bentuk kultur 3D spheroid. Penelitian ini menunjukkan adanya sinergisitas doxorubicin dan resveratrol pada dosis 60 µM yang terlihat pada presentase kematian sel, presentasi tutupan celah migrasi, dan respon spheroid sel SH-SY5Y. Kata kunci. Adjuvan, Kanker, Kemoterapi, Sinergisitas, Stress Oksidatif ***** Doxorubicin has long been a primary cancer chemotherapy drug. It has serious side effects when administered continuously, such as neurotoxicity through oxidative stress on normal nerve cells and peripheral neuropathy, due to the type of cell death not being entirely programmed cell death (apoptosis). The solution to these side effects is to combine doxorubicin with anticancer compounds that are low in toxicity to normal cells and compounds that predominantly direct cancer cell death to programmed cell death, such as resveratrol. This study aims to determine the potential of resveratrol as a chemotherapy adjuvant against doxorubicin through cytotoxicity levels, compound synergy, cell migration, and spheroid response to Neuroblastoma SH-SY5Y cancer cells. The treatment groups were divided into 0.005 µM doxorubicin, 40 µM resveratrol, 60 µM resveratrol, a combination of doxo+res 40 µM, a combination of doxo+res 60 µM, and a control group. The results of the study showed that the combination of doxorubicin and resveratrol synergistically killed SH-SY5Y cells, inhibited cell migration, and inhibited the proliferation and metastasis of SH-SY5Y cells in the form of 3D spheroid cultures. This study shows the synergy of doxorubicin and resveratrol at a dose of 60 µM which is seen in the percentage of cell death, presentation of migration gap closure, and spheroid response of SH-SY5Y cells. Keywords. Adjuvant, Cancer, Chemotherapy, Oxidative Stress, Synergy

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. ; 2). Prof. Dr. Yulia Irnidayanti, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Ilmu Kedokteran > Terapi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 30370 not found.
Date Deposited:	21 Aug 2025 03:37
Last Modified:	21 Aug 2025 03:37
URI:	http://repository.unj.ac.id/id/eprint/61738

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