PENGARUH RESVERATROL TERHADAP APOPTOSIS DAN NEKROSIS PADA SEL SH-SY5Y YANG DIINDUKSI DOXORUBICIN SECARA IN VITRO: ANALISIS ANNEXIN V

SHAFIYAH MARDIYAH, . (2025) PENGARUH RESVERATROL TERHADAP APOPTOSIS DAN NEKROSIS PADA SEL SH-SY5Y YANG DIINDUKSI DOXORUBICIN SECARA IN VITRO: ANALISIS ANNEXIN V. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Resveratrol merupakan senyawa polifenol yang diketahui memiliki efek proapoptotik dan antioksidan, serta berpotensi dikombinasikan dengan agen kemoterapi seperti doxorubicin untuk meningkatkan efektivitas terapi kanker. Penelitian ini bertujuan untuk mengetahui efek pemberian resveratrol dan doxorubicin terhadap kepadatan sel dan menganalisis perbedaan persentase pemberian resveratrol dan doxorubicin terhadap apoptosis dan nekrosis pada sel SH-SY5Y. Metode yang digunakan meliputi pengamatan mikroskopik dan analisis flow cytometry menggunakan Annexin V/Propidium Iodide (PI) selama 24 dan 48 jam. Hasil menunjukkan bahwa kombinasi RSV 60 µM + DOX secara signifikan menurunkan kepadatan sel dan meningkatkan apoptosis lanjut dibandingkan perlakuan RSV 40 µM +DOX. RSV dosis tinggi berperan sebagai prooksidan yang memicu stres oksidatif, sedangkan RSV dosis rendah bersifat antioksidan dan protektif. Meskipun kombinasi resveratrol dan doxorubicin meningkatkan apoptosis, kematian sel masih didominasi oleh nekrosis akibat stres oksidatif berlebih. Temuan ini menunjukkan bahwa resveratrol berpotensi meningkatkan efektivitas kemoterapi, namun optimalisasi dosis dan waktu perlu diperhatikan untuk meminimalkan efek samping nekrosis yang merugikan. Kata Kunci: Apoptosis, Doxorubicin, Nekrosis, Resveratrol, SH-SY5Y ***** Resveratrol (RSV) is a polyphenolic compound known to exert both pro-apoptotic and antioxidant effects, and has potential to be combined with chemotherapeutic agents such as doxorubicin (DOX) to enhance the effectiveness of cancer therapy. This study aimed to investigate the effects of RSV and DOX on cell density and to analyze the differences in the percentages of apoptosis and necrosis in SH-SY5Y cells following RSV and DOX treatment. The methods employed included microscopic observation and flow cytometry analysis using Annexin V/Propidium Iodide (PI) staining after 24 and 48 hours of incubation. The results demonstrated that the combination of RSV 60 µM + DOX significantly reduced cell density and increased late apoptosis compared to RSV 40 µM + DOX. High-dose RSV acted as a pro-oxidant, triggering oxidative stress, while low-dose RSV exhibited antioxidant and protective properties. Although the combination of RSV and DOX enhanced apoptosis, cell death was still predominantly necrotic, likely due to excessive oxidative stress. These findings suggest that RSV has the potential to enhance the efficacy of chemotherapy; however, dose and exposure time optimization is necessary to minimize necrosis-related side effects. Keywords: Apoptosis, Doxorubicin, Necrosis, Resveratrol, SH-SY5Y

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. ; 2). Prof. Dr. Yulia Irnidayanti, M.Si
Subjects:	Ilmu Kedokteran > Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan
Divisions:	FMIPA > S1 Biologi
Depositing User:	Shafiyah Mardiyah .
Date Deposited:	22 Aug 2025 03:13
Last Modified:	22 Aug 2025 03:13
URI:	http://repository.unj.ac.id/id/eprint/61880

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