IDENTIFIKASI DEL.11 GEN MYOSTATIN PADA SAPI LIMOUSIN JANTAN (BOS TAURUS) MENGGUNAKAN METODE PCR-RFLP

FASYA SAFFARA NABILA, . (2025) IDENTIFIKASI DEL.11 GEN MYOSTATIN PADA SAPI LIMOUSIN JANTAN (BOS TAURUS) MENGGUNAKAN METODE PCR-RFLP. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

	Text COVER.pdf Download (854kB)
	Text BAB I.pdf Download (305kB)
	Text BAB II.pdf Restricted to Registered users only Download (303kB) | Request a copy
	Text BAB III.pdf Restricted to Registered users only Download (352kB) | Request a copy
	Text BAB IV.pdf Restricted to Registered users only Download (440kB) | Request a copy
	Text BAB V.pdf Restricted to Registered users only Download (204kB) | Request a copy
	Text DAFTAR PUSTAKA.pdf Download (299kB)
	Text LAMPIRAN.pdf Restricted to Registered users only Download (799kB) | Request a copy

Abstract

Gen myostatin merupakan gen pengatur utama perkembangan otot rangka dan dikenal sebagai penghambat pertumbuhan otot. Mutasi delesi 11 basa pada ekson 3 gen myostatin diketahui menyebabkan hilangnya fungsi protein Myostatin, sehingga meningkatkan massa otot rangka (double muscling). Penelitian ini bertujuan untuk mengidentifikasi keberadaan mutasi del.11 pada gen myostatin dan menghitung frekuensi alel serta nilai heterozigositas pada populasi sapi Limousin Jantan asal BBIB Lembang dan Singosari. Metode yang digunakan pada penelitian ini adalah metode kuantitatif dengan pendekatan deskriptif. Sebanyak 30 sampel DNA dari semen beku sapi Limousin jantan dianalisis dengan primer spesifik myostatin dan enzim restriksi NmuCI (Tsp45I) menggunakan Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) kemudian dihitung frekuensi genotipe, frekuensi alel, serta nilai heterozigositas pengamatan (Ho) dan heterozigositas harapan (He). Hasil elektroforesis menunjukkan semua sampel memiliki satu pita DNA berukuran 451 bp, yang mengindikasikan genotipe homozigot normal (+/+). Genotipe heterozigot (+/del.11) maupun homozigot mutan (del.11/del.11) tidak ditemukan pada populasi yang diteliti. Hasil ini diperkuat melalui analisis sekuensing. Frekuensi alel del.11 sebesar 0 dan alel normal sebesar 1, sehingga populasi bersifat monomorfik dengan nilai heterozigositas pengamatan (Ho) dan harapan (He) sebesar 0. Hasil ini menunjukkan bahwa mutasi del.11 tidak ditemukan pada populasi sapi Limousin jantan yang diteliti. ***** The myostatin gene is a key regulator of skeletal muscle development and is known to inhibit muscle growth. An 11-base deletion mutation in exon 3 of the myostatin gene is known to cause loss of Myostatin protein function, resulting in increased skeletal muscle mass (double muscling). This study aimed to identify the presence of the del.11 mutation in the myostatin gene and to calculate the allele frequency and heterozygosity values in a population of male Limousin cattle from BBIB Lembang and Singosari. The method used in this study was a quantitative method with a descriptive approach. A total of 30 DNA samples from frozen semen of male Limousin cattle were analyzed with myostatin specific primers and the restriction enzyme NmuCI (Tsp45I) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) then the genotype frequency, allele frequency, observed heterozygosity (Ho) and expected heterozygosity (He) values were calculated. Electrophoresis results showed that all samples had one DNA band measuring 451 bp, indicating a normal homozygous genotype (+/+). Neither heterozygous (+/del.11) nor homozygous mutant (del.11/del.11) genotypes were found in the studied population. This result was confirmed by sequencing analysis. The frequency of the del.11 allele was 0 and the normal allele was 1, indicating that the population was monomorphic with observed (Ho) and expected (He) heterozygosity values of 0. These results indicate that the del.11 mutation was not found in the studied male Limousin cattle population.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Prof. Dr. Yulia Irnidayanti, M.Si. ; 2). Dr. Sutikno, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam
Divisions:	FMIPA > S1 Biologi
Depositing User:	Fasya Saffara Nabila .
Date Deposited:	27 Aug 2025 04:16
Last Modified:	27 Aug 2025 04:16
URI:	http://repository.unj.ac.id/id/eprint/62071

Actions (login required)

View Item