DEKOLORISASI ZAT WARNA SINTETIS METHYLENE BLUE MENGGUNAKAN KAPANG DARK SEPTATE ENDOPHYTE DAN LIGHT EXPANDED CLAY AGGREGATE UNTUK BIOREMEDIASI SKALA LABORATORIUM

NELLA ANGGITA, . (2025) DEKOLORISASI ZAT WARNA SINTETIS METHYLENE BLUE MENGGUNAKAN KAPANG DARK SEPTATE ENDOPHYTE DAN LIGHT EXPANDED CLAY AGGREGATE UNTUK BIOREMEDIASI SKALA LABORATORIUM. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Pencemaran air dari pembuangan limbah yang mengandung zat warna sintetis seperti Methylene blue menjadi perhatian serius karena sifatnya yang toksik dan sulit terurai secara alami. Penelitian ini bertujuan untuk mengevaluasi kemampuan kapang Dark Septate Endophyte dalam mendegradasi Methylene blue pada media padat dan cair, menilai pengaruh kondisi shaker dan non-shaker, serta efektivitas imobilisasi DSE menggunakan media Light Expanded Clay Aggregate (LECA) pada berbagai konsentrasi zat warna. Metode penelitian dilakukan secara eksperimental menggunakan analisis ANOVA satu arah dan uji lanjut Tukey. Isolat yang digunakan terdiri dari 3 isolat yaitu KSP, PP dan AM3 yang merupakan hasil isolasi dan koleksi dari Badan Riset dan Inovasi Nasional (BRIN). Hasil menunjukkan bahwa strain DSE mampu mendekolorisasi MB pada media padat dan media cair, dengan kemampuan tertinggi ditunjukkan oleh strain AM3 yaitu indeks dekolorisasi sebesar 1,6 pada media padat dan kemampuan dekolorisasi tertinggi oleh strain AM3 yaitu sebesar 67,27% pada media cair. Perlakuan shaker meningkatkan efektivitas dekolorisasi, hasil pada strain AM3 menunjukkan aktivitas tertinggi pada kondisi shaker oleh AM3 sebesar 67,27%, sedangkan strain KSP menunjukkan aktivitas tertinggi sebesar 46,09% pada kondisi non-shaker. Pada perlakuan berbagai konsentrasi zat warna, strain AM3 menunjukkan efisiensi dekolorisasi tertinggi sebesar 95,98% pada konsentrasi 25 ppm dalam bentuk sel bebas, dan efesiensi dekolorisasi tertinggi sebesar 70,71% pada konsentrasi 25 ppm dalam kondisi terimobilisasi pada LECA. Kata Kunci: bioremediasi, imobiliasi, dekolorisasi, LECA, methylene blue, dark septate endophyte. ***** Water pollution from the discharge of waste containing synthetic dyes such as methylene blue is a serious concern due to its toxic nature and difficulty in breaking down naturally. This study aims to evaluate the ability of Dark Septate Endophyte fungi to degrade Methylene blue in solid and liquid media, assess the influence of shaker and non-shaker conditions, and determine the effectiveness of DSE immobilization using Light Expanded Clay Aggregate (LECA) media at various dye concentrations. The research method was conducted experimentally using one-way ANOVA analysis and Tukey's post-hoc test. The isolates used consisted of three isolates, namely KSP, PP, and AM3, which were the results of isolation and collection from the National Research and Innovation Agency (BRIN). The results showed that DSE strains were capable of decolorizing MB in both solid and liquid media, with the highest decolorization index of 1.6 in solid media demonstrated by strain AM3, and the highest decolorization efficiency of 67.27% in liquid media also by strain AM3. Shaker treatment enhanced decolorization efficiency; results for strain AM3 showed the highest activity under shaker conditions at 67.27%, while strain KSP exhibited the highest activity at 46.09% under non-shaker conditions. Under various dye concentration treatments, strain AM3 showed the highest decolorization efficiency of 95.98% at a concentration of 25 ppm in free-form cells, and the highest decolorization efficiency of 70.71% at a concentration of 25 ppm under immobilized conditions on LECA. Keywords: bioremediation, immobilization, decolorization, LECA, methylene blue, Dark septate endophyte.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Prof.Dr. Ratna Komala, M.Si. ; 2). Dr. Irma Melati, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi > Biokimia
Divisions:	FMIPA > S1 Biologi
Depositing User:	Nella Anggita .
Date Deposited:	27 Aug 2025 06:34
Last Modified:	27 Aug 2025 06:34
URI:	http://repository.unj.ac.id/id/eprint/62126

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