MIKROPROPAGASI DAN PROFIL METABOLIT SEKUNDER Rubus moluccanus L. MELALUI VARIASI BAP (Benzyl Amino Purine) DAN NAA (Naphthalene Acetic Acid)

ANGGUN PRASIWI, . (2026) MIKROPROPAGASI DAN PROFIL METABOLIT SEKUNDER Rubus moluccanus L. MELALUI VARIASI BAP (Benzyl Amino Purine) DAN NAA (Naphthalene Acetic Acid). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Ketersediaan populasi Rubus moluccanus sebagai sumber senyawa bioaktif di alam terkendala oleh laju pertumbuhan yang lambat. Penelitian ini bertujuan untuk mengoptimalkan perbanyakan melalui kultur in vitro menggunakan kombinasi zat pengatur tumbuh (ZPT) Benzyl Amino Purine (BAP) dan Naphthalene Acetic Acid (NAA), serta menganalisis profil metabolit sekundernya. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) nonfaktorial dengan tujuh variasi konsentrasi BAP dan NAA. Parameter yang diamati pada tahap inisiasi meliputi hari muncul tunas, jumlah tunas, jumlah daun, dan tinggi tunas. Sementara itu, parameter pada tahap subkultur mencakup jumlah tunas, jumlah daun, tinggi tunas, serta berat basah dan berat kering. Karakterisasi profil metabolit sekunder dilakukan melalui analisis rendemen dan uji Gas Chromatography-Mass Spectrometry (GC-MS). Hasil penelitian menunjukkan bahwa kombinasi ZPT memberikan pengaruh signifikan pada pertumbuhan tunas. Pada tahap inisiasi, perlakuan tanpa ZPT memberikan hasil terbaik dengan menghasilkan jumlah tunas, daun, dan tinggi tunas tertinggi. Hal ini mengindikasikan dominasi hormon endogen pada fase awal. Namun, pada tahap subkultur, penambahan ZPT eksogen menjadi faktor determinan, dengan kombinasi 1,5 mg/L BAP + 0,2 mg/L NAA memberikan hasil tertinggi pada seluruh parameter pertumbuhan dan akumulasi biomassa secara signifikan. Analisis GC-MS mengidentifikasi 57 senyawa pada perlakuan tanpa ZPT, sedangkan perlakuan 1 mg/L BAP + 0,5 mg/L NAA dan 1,5 mg/L BAP + 0,2 mg/L NAA masing-masing hanya mengidentifikasi 29 dan 31 senyawa. Temuan ini menunjukkan bahwa keberadaan ZPT memengaruhi dan memodifikasi jalur biosintesis metabolit sekunder.***** The availability of Rubus moluccanus populations as a source of bioactive compounds in nature is constrained by their slow growth rate. This study aimed to optimize plant propagation through in vitro culture using a combination of plant growth regulators (PGRs), namely Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA), and to analyze their secondary metabolite profiles. The research employed a nonfactorial Completely Randomized Design (CRD) with seven variations of BAP and NAA concentrations. Parameters observed during the initiation stage included the time of shoot emergence, number of shoots, number of leaves, and shoot height. Meanwhile, parameters at the subculture stage encompassed the number of shoots, number of leaves, shoot height, as well as fresh and dry weight. Characterization of secondary metabolite profiles was conducted through yield analysis and Gas Chromatography-Mass Spectrometry (GC-MS). The results indicated that the combination of PGRs significantly influenced shoot growth. During the initiation stage, the treatment without PGRs yielded the best results, producing the highest number of shoots, leaves, and shoot height. This indicates the dominance of endogenous hormones during the early phase. However, at the subculture stage, the addition of exogenous PGRs became a determinant factor, where the combination of 1,5 mg/L BAP + 0,2 mg/L NAA significantly produced the highest results across all growth parameters and biomass accumulation. GC-MS analysis identified 57 compounds in the treatment without PGRs, whereas the treatments with 1 mg/L BAP + 0,5 mg/L NAA and 1,5 mg/L BAP + 0,2 mg/L NAA identified only 29 and 31 compounds, respectively. These findings suggest that the presence of PGRs influences and modifies the biosynthetic pathways of secondary metabolites in R. moluccanus.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Ns. Sri Rahayu, M.Biomed. 2). Lily Ismaini, M.Si.
Subjects:	Sains > Ilmu Bumi > Biologi Sains > Botani Sains > Fisiologi Sains > Mikro Biologi > Biokimia Ilmu Kedokteran > farmasi Teknologi dan Ilmu Terapan > Teknologi (umum)
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 32709 not found.
Date Deposited:	04 Feb 2026 04:25
Last Modified:	04 Feb 2026 04:25
URI:	http://repository.unj.ac.id/id/eprint/64840

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