UJI TOKSISITAS DAN PROFIL HISTOLOGIS TESTIS MENCIT JANTAN (Mus musculus) PASCA PEMBERIAN EKSTRAK KEDELAI IRADIASI (Glycine max (L.) Merrill.) SUGENTAN I

KAMAL MUHAMMAD JAWWAD K, . (2026) UJI TOKSISITAS DAN PROFIL HISTOLOGIS TESTIS MENCIT JANTAN (Mus musculus) PASCA PEMBERIAN EKSTRAK KEDELAI IRADIASI (Glycine max (L.) Merrill.) SUGENTAN I. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

	Text COVER.pdf Download (620kB)
	Text BAB 1.pdf Download (266kB)
	Text BAB 2.pdf Restricted to Registered users only Download (409kB) | Request a copy
	Text BAB 3.pdf Restricted to Registered users only Download (363kB) | Request a copy
	Text BAB 4.pdf Restricted to Registered users only Download (411kB) | Request a copy
	Text BAB 5.pdf Restricted to Registered users only Download (258kB) | Request a copy
	Text Daftar Pustaka.pdf Download (243kB)
	Text Lampiran.pdf Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Indonesia sebagai negara dengan basis pertanian memiliki peluang besar dalam pengembangan tanaman kedelai sebagai sumber utama protein nabati. Salah satu varietas unggul hasil teknologi iradiasi yang dikembangkan oleh Badan Tenaga Nuklir Nasional (BATAN) adalah Sugentan I, memiliki masa panen singkat dan produksi yang tinggi. Kedelai juga mengandung metabolit sekunder, khususnya isoflavon, yang mempunyai aktivitas biologis dan berpotensi memengaruhi struktur histologis testis. Penelitian ini bertujuan untuk menilai tingkat toksisitas ekstrak kedelai Sugentan I serta pengaruhnya terhadap gambaran histologis testis. Uji toksisitas ekstrak dilakukan menggunakan metode Brine Shrimp Lethality Test (BSLT) untuk menentukan nilai LC₅₀. Pengaruh ekstrak terhadap profil histologi testis dianalisis melalui pengamatan berat badan, berat testis, diameter tubulus seminiferus, jumlah sel spermatogonium, dan jumlah sel spermatosit mencit. Data yang diperoleh dianalisis secara statistik menggunakan uji one-way ANOVA dengan uji lanjut pada tingkat kepercayaan 95% (α = 0,05). Hasil penelitian menunjukkan bahwa ekstrak kedelai Sugentan I maupun kedelai impor termasuk dalam kategori tidak toksik dengan nilai LC₅₀ masing-masing sebesar 1270,42 ppm dan 1039,08 ppm. Pemberian ekstrak kedelai Sugentan I dan ekstrak kedelai impor tidak memberikan pengaruh terhadap berat badan dan berat testis mencit, namun ekstrak kedelai Sugentan I mampu meningkatkan diameter tubulus seminiferus serta jumlah sel spermatogonium dan spermatosit mencit jantan.*****Indonesia, as an agriculture-based country, has significant potential for the development of soybean as a primary source of plant protein. One superior variety produced through irradiation technology by the National Nuclear Energy Agency (BATAN) is Sugentan I, which has a short harvesting period and high yield. Soybean also contains secondary metabolites, particularly isoflavones, which possess biological activity and may affect the histological structure of the testes. This study aimed to assess the toxicity level of Sugentan I soybean extract and its effects on the histological appearance of the testes. Toxicity testing was conducted using the Brine Shrimp Lethality Test (BSLT) method to determine the LC₅₀ value. The effect of extract administration on the testicular histological profile was evaluated by observing body weight, testicular weight, seminiferous tubule diameter, the number of spermatogonia cells, and the number of spermatocyte cells in mice. The data obtained were statistically analyzed using a one-way ANOVA followed by a post-hoc test at a 95% confidence level (α = 0.05). The results showed that both Sugentan I soybean extract and imported soybean extract were categorized as non-toxic, with LC₅₀ values of 1270.42 ppm and 1039.08 ppm, respectively. Administration of Sugentan I soybean extract and imported soybean extract did not affect body weight or testicular weight in mice; however, Sugentan I soybean extract increased the diameter of the seminiferous tubules as well as the number of spermatogonia and spermatocyte cells in male mice.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Elsa Lisanti, M.Si. ; 2). Ns. Sri Rahayu, S.Kep., M.Biomed.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam
Divisions:	FMIPA > S1 Biologi
Depositing User:	Kamal Muhammad Jawwad K .
Date Deposited:	20 Feb 2026 03:13
Last Modified:	20 Feb 2026 03:13
URI:	http://repository.unj.ac.id/id/eprint/65545

Actions (login required)

View Item