PENGUJIAN AKTIVITAS ANTIMIKROBA DAN ANTIOKSIDAN KAPANG ENDOFIT ASAL AKAR TANAMAN TEBU (SACCHARUM OFFICINARUM) DESA REJOSARI LAMPUNG

RAPIKA SARI, . (2022) PENGUJIAN AKTIVITAS ANTIMIKROBA DAN ANTIOKSIDAN KAPANG ENDOFIT ASAL AKAR TANAMAN TEBU (SACCHARUM OFFICINARUM) DESA REJOSARI LAMPUNG. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Sumber daya alam yang beraneka ragam dapat berpotensi menghasilkan senyawa antimikroba dan antioksidan. Penelitian ini bertujuan untuk menguji aktivitas antimikroba dan antioksidan kapang endofit koleksi UNJCC asal akar tanaman tebu. Metode deskriptif dilakukan pada identifikasi kapang berdasarkan daerah rDNA Internal Transcribed Spacer dan karakterisasi morfologi kapang. Metode eksperimen digunakan untuk uji aktivitas antimikroba dan antioksidan, dengan menggunakan Rancangan Acak Lengkap (RAL) faktorial. Pengujian aktivitas antimikroba digunakan dua faktor yaitu jenis kapang endofit (UNJCC F24, F25 dan F26) dan jenis mikroba uji (L. monocytogenes, S. enteritidis, dan C. albicans), parameter uji berupa diameter zona hambat. Pengujian aktivitas antioksidan digunakan dua faktor yaitu jenis kapang endofit (UNJCC F24, F25 dan F26) dan konsentrasi supernatan kapang endofit (1.000, 2.000, dan 3.000 ppm) dengan menggunakan metode peredaman radikal DPPH, parameter uji berupa persen inhibisi DPPH. Data dianalisis menggunakan ANAVA dua arah univariate dilanjutkan dengan diuji Duncan Multiple Range Test (DMRT) 5%. Hasil identifikasi molekular dan karakterisasi morfologi menunjukkan isolat UNJCC F24, F25, dan F26 teridentifikasi berturut-turut sebagai A. terreus (homologi 98,38%), P. javanicum (homologi 99,64%), dan P. singorense (homologi 100%). Hasil pengujian aktivitas antimikroba menunjukkan kapang endofit P. javanicum UNJCC F25 mampu menghambat L. monocytogenes, S. enteritidis, dan C. albicans. Kapang endofit A. terreus UNJCC F24 dan P. singorense UNJCC F26 hanya mampu menghambat L. monocytogenes dan S. enteritidis. Hasil pengujian aktivitas antimikroba menunjukkan nilai zona hambat tertinggi dihasilkan oleh P. javanicum UNJCC F25 terhadap C. albicans sebesar 8,92 mm. Hasil pengujian aktivitas antioksidan menunjukkan seluruh kapang endofit dapat meredam radikal bebas DPPH, dengan persen inhibisi tertinggi dihasilkan oleh A. terreus UNJCC F24 pada kosentrasi 3.000 ppm sebesar 62,81%. Berdasarkan hasil penelitian, P. javanicum UNJCC F25 memiliki aktivitas antimikroba paling tinggi sehingga berpotensi sebagai sumber bahan alami penghasil senyawa antimikroba dan A. terreus UNJCC F24 memiliki aktivitas antioksidan paling tinggi sehingga berpotensi sebagai sumber bahan alami penghasil senyawa antioksidan. ********** Diverse natural resources can potentially produce antimicrobial and antioxidant compounds. This study aimed to examine the antimicrobial and antioxidant activity of endophytic fungal from the UNJCC from the roots of the sugarcane plant. The descriptive method was carried out on the identification of fungal based on the rDNA Internal Transcribed Spacer region and the morphological characterization of the fungal. The experimental method was used to test the antimicrobial and antioxidant activity, using a factorial Completely Randomized Design (CRD). The antimicrobial activity test used two factors, namely the type of endophytic fungi (UNJCC F24, F25 and F26) and the type of microbe tested (L. monocytogenes, S. enteritidis, and C. albicans), the diameter of the inhibition zone was used as a parameter. The antioxidant activity test used two factors, namely the type of endophytic fungi (UNJCC F24, F25 and F26) and the supernatant concentration of endophytic fungi (1.000, 2.000, and 3.000 ppm) using the DPPH radical reduction method, the percent inhibition of DPPH was used as a parameter. Data were analyzed using univariate two-way ANOVA followed by Duncan Multiple Range Test (DMRT) 5%. The results of molecular identification and morphological characterization showed that the isolates UNJCC F24, F25, and F26 were identified as A. terreus (98.38%), P. javanicum (99.64%), and P. singorense (100%). The antimicrobial activity test results showed that the endophytic fungal P. javanicum UNJCC F25 was able to inhibit L. monocytogenes, S. enteritidis, and C. albicans. Endophytic fungal A. terreus UNJCC F24 and P. singorense UNJCC F26 were only able to inhibit L. monocytogenes and S. enteritidis. Antimicrobial activity test showed that the highest inhibition zone value was produced by P. javanicum UNJCC F25 against the pathogenic yeast C. albicans of 8.92 mm. The results of the antioxidant activity test showed that all fungal endophyte could reduce DPPH free radicals, with the highest percentage of inhibition produced by A. terreus UNJCC F24 at a concentration of 3.000 ppm at 62.81%. Based on the results of the study, P. javanicum UNJCC F25 has the highest potential as a natural source of producers of antimicrobial compounds and A. terreus UNJCC F24 has the highest potential as a natural source of producing antioxidant compounds.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Dalia Sukmawati, M.Si.; 2). Ns. Sri Rahayu, S.Kep., M.Biomed.
Subjects:	Sains > Ilmu Bumi > Biologi Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 13831 not found.
Date Deposited:	06 Mar 2022 09:33
Last Modified:	06 Mar 2022 09:33
URI:	http://repository.unj.ac.id/id/eprint/23330

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