POTENSI BAKTERI Pseudomonas lactis SEBAGAI PENGENDALI HAYATI PENYAKIT BUSUK HITAM PADA PAKCOY YANG DISEBABKAN PATOGEN Xanthomonas campestris pv. campestris

SITI MARLINIA ARINI, . (2024) POTENSI BAKTERI Pseudomonas lactis SEBAGAI PENGENDALI HAYATI PENYAKIT BUSUK HITAM PADA PAKCOY YANG DISEBABKAN PATOGEN Xanthomonas campestris pv. campestris. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Xanthomonas campestris pv. campestris penyebab penyakit busuk hitam merupakan patogen utama yang menyebabkan kegagalan panen tanaman Brassica. Pengendalian patogen ini umumnya menggunakan pestisida kimia berbahan dasar tembaga dan antibiotik. Namun, keduanya menimbulkan efek negatif bagi lingkungan. Penelitian ini bertujuan untuk mengetahui potensi bakteri Pseudomonas lactis sebagai pengendali alternatif penyakit busuk hitam pada tanaman pakcoy (Brassica rapa subsp. chinensis). Metode yang digunakan adalah deskriptif dan eksperimental. Beberapa tahap pengujian yang dilakukan meliputi uji antagonistik P. lactis terhadap patogen, uji hipersensitivitas P. lactis, uji patogenisitas X. campestris pv. campestris, dan uji efektivitas P. lactis dalam menghambat penyakit busuk hitam pada tanaman pakcoy. Hasil pengujian antagonisme in vitro didapati bahwa ketiga strain P. lactis yang digunakan (EA1, EB4, EB6) mampu menciptakan zona hambat terhadap patogen X. campestris pv. campestris. Ketiga strain tersebut juga tidak menimbulkan reaksi hipersensitif pada tanaman pakcoy, sehingga dapat digunakan sebagai agen untuk menghambat pertumbuhan patogen. Hasil uji patogenisitas menunjukkan bahwa patogen yang digunakan memiliki virulensi paling baik pada konsentrasi 109. Hasil uji di rumah kaca menunjukkan bahwa P. lactis EB4 lebih efektif dalam menghambat penyakit busuk hitam pada tanaman pakcoy varietas Nauli (61,1 – 61,6%). Pada tanaman pakcoy varietas Flamingo P. lactis EA1 cukup efektif menghambat busuk hitam (54,6 – 58,8%). Tanaman pakcoy terinfeksi patogen yang diberi P. lactis EA1 secara umum menunjukkan respon pertumbuhan paling baik berdasarkan tinggi tanaman, jumlah daun, dan luas daun. Kata kunci: biokontrol, black rot, Brassicaceae, patogen tumbuhan. Xanthomonas campestrispv. campestris, which causes black rot, is the primary pathogen that causes Brassica crop failure. Control of this pathogen generally uses copper-based pesticides and antibiotics. However, both have adverse effects on the environment. This research aims to determine the potential of Pseudomonas lactis as an alternative control of black rot disease on pakcoy plants (Brassica rapa subsp. chinensis). The method used is descriptive and experimental. Several stages of testing were carried out, including antagonism tests of P. lactis against pathogens, hypersensitivity test of P. lactis, and pathogenicity test of X. campestris pv. campestris, and test the effectiveness of P.lactisin in inhibiting black rot disease on pakcoy plants. The results of in vitro antagonism testing showed that the three strains of P. lactis (EA1, EB4, EB6) could create an inhibition zone against the pathogen X. campestris pv. campestris. These three strains also do not cause hypersensitive reactions in pakcoy plants, so they can be used as agents to inhibit the growth of pathogens. The results of the pathogenicity test showed that the pathogen used had the best virulence at a concentration of 109. The test results in the greenhouse showed that P. lactis EB4 was more effective in inhibiting black rot disease on Nauli variety pakcoy plants (61.1 – 61.6%). In Flamingo variety pakcoy plants, P.lactis EA1 inhibited black rot (54.6 – 58.8%). Pakcoy plants infected with pathogens were given P.lactis EA1, which generally showed the best growth response in plant height, number of leaves and leaf area. Keyword: biocontrol, black rot, Brassicaceae, plant pathogens.

Item Type:	Thesis (Sarjana)
Additional Information:	1) Dr. Reni Indrayanti, M.Si. 2) Rizal Koen Asharo, M.Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Botani Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Siti Marlinia Arini .
Date Deposited:	02 Aug 2024 01:45
Last Modified:	02 Aug 2024 01:45
URI:	http://repository.unj.ac.id/id/eprint/47924

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