ISOLASI KHAMIR POTENSIAL PROBIOTIK ASAL SALURAN PENCERNAAN LEBAH MADU (Apis mellifera)

SHOFFIA MEDINA AL HAQ, . (2024) ISOLASI KHAMIR POTENSIAL PROBIOTIK ASAL SALURAN PENCERNAAN LEBAH MADU (Apis mellifera). Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Probiotik merupakan mikroorganisme hidup yang memberikan manfaat jika diberikan dalam jumlah yang cukup. Khamir dapat ditemukan bergantung pada sumber gula, seperti halnya serangga pengonsumsi makanan yang terdapat sumber gula yang salah satunya adalah lebah madu (Apis mellifera). Penelitian ini bertujuan untuk mendapatkan isolat khamir asal saluran pencernaan lebah yang potensial sebagai agen probiotik serta mendapatkan identitas khamir tersebut. Pengujian probiotik terdiri dari uji toleransi garam empedu (0%, 0,5%, 1%, 1,5%, dan 2%); toleransi asam lambung pada pH 2 (variasi waktu inkubasi 0, 3, 6, dan 9 jam); aktivitas antibakteri terhadap Listeria monocytogenes dan Salmonella entiritidis. Desain penelitian menggunakan Rancangan Acak Lengkap (RAL). Data dianalisis menggunakan anava dua arah kemudian dilanjutkan uji Duncan Multiple Range Test (DMRT) 5%. Diperoleh 11 isolat dari hasil isolasi khamir. Skrining PBS pH 2 dipilih empat isolat khamir untuk pengujian lanjut uji probiotik yaitu L5.3 (UNJCC Y-172), L9.3 (UNJCC Y-173), L12.4 (UNJCC Y-174), dan L20.2 (UNJCC Y-175). Hasil identifikasi daerah D1/D2 rDNA diperoleh identitas keempat khamir adalah Starmerella potacharoeniae dengan tingkat homologi sebesar 99,11% dan bootstrap 93%. Pengujian garam empedu dan asam lambung menunjukan seluruh isolat uji mampu tumbuh hingga konsentrasi 2% dan waktu inkubasi 9 jam dengan persentase bertahan hidup >70%. Pengujian aktivitas antibakteri menunjukkan bahwa seluruh khamir uji kecuali Starmerella potacharoeniae UNJCC Y-174 mampu menghambat pertumbuhan bakteri Salmonella entiritidis dan seluruh isolat tidak dapat menghambat pertumbuhan Listeria monocytogenes. Kata Kunci: probiotik, khamir, Apis mellifera. ***** Probiotics are live microorganisms that provide benefits if given in sufficient quantities. Yeast can be found depending on the source of sugar, as well as insects that consume food containing sugar sources, one of which is honey bees (Apis mellifera). This study aims to obtain isolates of yeast from the digestive tract of bees that are potential as probiotic agents and obtain the identity of these yeasts. Probiotic testing consisted of bile salt tolerance test (0%, 0.5%, 1%, 1.5%, and 2%); gastric acid tolerance at pH 2 (incubation time variation of 0, 3, 6, and 9 hours); antibacterial activity against Listeria monocytogenes and Salmonella entiritidis. The research design used a completely randomized design (RAL). Data were analyzed using two-way ANOVA followed by Duncan Multiple Range Test (DMRT) 5%. Obtained 11 isolates from the isolation of yeast. Screening PBS pH 2 selected four yeast isolates for further testing probiotic test, namely L5.3 (UNJCC Y-172), L9.3 (UNJCC Y-173), L12.4 (UNJCC Y-174), and L20.2 (UNJCC Y-175). The results of the identification of the D1/D2 rDNA region obtained the identity of the four yeasts is Starmerella potacharoeniae with a homology level of 99.11% and 93% bootstrap. Bile salt and gastric acid testing showed that all test isolates were able to grow up to a concentration of 2% and an incubation time of 9 hours with a survival percentage of >70%. Antibacterial activity testing showed that all test yeasts except Starmerella potacharoeniae UNJCC Y-174 were able to inhibit the growth of Salmonella entiritidis bacteria and all isolates could not inhibit the growth of Listeria monocytogenes. Keywords: probiotic, yeast, Apis mellifera

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Dalia Sukmawati, M.Si. 2). Rizal Koen Asharo, S,Si., M,Si.
Subjects:	Sains > Sains, Ilmu Pengetahuan Alam Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Users 25125 not found.
Date Deposited:	09 Aug 2024 06:06
Last Modified:	09 Aug 2024 06:06
URI:	http://repository.unj.ac.id/id/eprint/49538

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