BIODEGRADASI FENANTRENA DENGAN MENGGUNAKAN JAMUR LAUT ASAL PESISIR BALIKPAPAN

RAHMAH KHAIRUNNISA QONITA, . (2025) BIODEGRADASI FENANTRENA DENGAN MENGGUNAKAN JAMUR LAUT ASAL PESISIR BALIKPAPAN. Sarjana thesis, UNIVERSITAS NEGERI JAKARTA.

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Abstract

Pencemaran laut di Indonesia yang disebabkan oleh senyawa PAH (Poliaromatik Hidrokarbon) merupakan masalah yang serius. Fenantrena sebagai salah satu jenis PAH sering menjadi fokus penelitian karena sering ditemukan di lingkungan. Kelompok jamur sebagai mikroorganisme yang diketahui mampu memproduksi enzim lignolitik untuk mendegradasi fenantrena. Penelitian ini dilakukan untuk menentukan kemampuan isolat jamur laut dari Pesisir Balikpapan, Kalimantan Timur, dalam mendegradasi fenantrena. Metode yang digunakan dalam penelitian ini adalah deskriptif dan eksperimental, yang dianalisis dengan MANOVA dua arah dan tiga arah. Hasil penelitian menunjukkan bahwa FPB21 dan FPB23 menunjukkan potensinya dalam mendegradasi fenantrena. Hasil MANOVA dari interaksi isolat FPB21 yang diinkubasi selama 28 hari (62,82%) mampu menghasilkan persentase degradasi fenantrena tertinggi. Biomassa tertinggi diperoleh pada hari ke-14 inkubasi (15,5 g/L). Kombinasi isolat FPB21 pada pH 7 dengan inkubasi selama 28 hari menghasilkan persentase degradasi tertinggi (80,86%) dan peningkatan biomassa tertinggi dalam interaksi FPB21 pada pH 7 (13,94 g/L). Oleh karena itu, pH 7 merupakan nilai optimal untuk proses degradasi fenantrene. Sumber nitrogen amonium nitrat menghasilkan persentase degradasi tertinggi (99,17%). Biomassa tertinggi (21,5 g/L) diamati pada FPB21 dengan sumber nitrogen ekstrak ragi selama 28 hari inkubasi. Penelitian ini membuktikan jamur laut FPB21 dan FPB23 mampu mendegradasi phenanthrene secara efektif. Nilai pH 7 dan ammonium nitrat adalah kondisi terbaik untuk degradasi, sementara yeast extract mendukung pertumbuhan biomassa. Hasil ini mendukung pengembangan bioremediasi ramah lingkungan. ***** Marine pollution in Indonesia caused by PAH (Polycyclic Aromatic Hydrocarbon) compounds is a serious problem. Phenantrhene as a type of PAH is often the focus of research because it is often found in the environment. Fungi as microorganisms, are known to have the ability to produce lignolytic enzymes to degrade phenanthrene. This study was conducted to determine the ability of marine mushroom isolate from the Balikpapan Coast, East Kalimantan, to degrade phenanthrene. The methods used in this study were descriptive and experimental, which were analyzed with two-way and three-way MANOVA. The results showed that FPB21 and FPB23 showed their potential in degrading phenanthrene. MANOVA results from the interaction of FPB21 isolates incubated for 28 days (62.82%) were able to produce the highest percentage of phenanthrene degradation. The highest biomass was obtained on the 14th day of incubation (15.5 g/L). The combination of FPB21 isolates at pH 7 with incubation for 28 days resulted in the highest percentage of degradation (80.86%) and the highest biomass increase in FPB21 interactions at pH 7 (13.94 g/L ). Therefore, pH 7 is the optimal value for the phenanthrene degradation process. The nitrogen source of ammonium nitrate produces the highest percentage of degradation (99.17%). The highest biomass (21.5 g/L) was observed at FPB21 with a nitrogen source of yeast extract during 28 days of incubation. This study proves that the sea mushrooms FPB21 and FPB23 are able to degrade phenanthrene effectively. A pH value of 7 and ammonium nitrate are the best conditions for degradation, while yeast extract supports biomass growth. These results support the development of environmentally friendly bioremediation.

Item Type:	Thesis (Sarjana)
Additional Information:	1). Dr. Tri Handayani Kurniati, M.Si. ; 2). Risky Ayu Kristanti, P.hD., Eng.
Subjects:	Sains > Mikro Biologi
Divisions:	FMIPA > S1 Biologi
Depositing User:	Rahmah Khairunnisa Qonita .
Date Deposited:	29 Aug 2025 03:23
Last Modified:	29 Aug 2025 03:23
URI:	http://repository.unj.ac.id/id/eprint/62242

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